Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Detection and typing of human papilloma virus DNA in cervical cancer with In situ hybridization method

Authors
Mohammad Niakan 1
Ahya Garshasbi 2
Mohammad Reza Jalali 3
Modares Gilani 4
Soghrat Faghihzadeh 5
1 Microbiology Department, Faculty of Medicine, Shahed University, Tehran, Iran
2 Department of Obstet . and Gynecol Of Medicine, Shahed University, Tehran, Iran
3 Pathology Department, Faculty of Medicine, Shahed University, Tehran, Iran
4 Department of Gynecology and Obstetrics ,Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5 Department of Biostatistics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Cervical cancer is one of the most frequently found cancers in women and appears to have a viral aetiology.certain types of the human papillomavirus (HPV) are well established as the primary causes of cervical cancer.Clinical follow-up data, histopathologic diagnosis, Insitu hybridization (ISH ) and HPV DNA typing were available from 60 patients.ISH technique was performed with comercial biotinylated probes.The presence of 7 high risk HPV was evaluated in 60 cervical biopsies with squamous cell carcinoma (SCC) and Cervical Intraepithelial neoplasia (CIN) of differeat degrees by ISH.We analysed 60 biopsies from Iranian women. 42 of 60 (70%) carcinoma specimens were positive for HPV-DNA. HPV 31/33/51 (25%) was most frequently found, follwed by HPV 16/18 (23.33%) and HPV 6/11 (21.66%) while HPV negative cases were 18(30%).High risk HPV types appear to be most frequently associated with SCC and CIN. ISH is a sensitive test in the detection and typing of HPV DNA both in clinical and latent infections.
Keywords
Cervical cancer
human papillomavirus
Insitu hybridization
Pathology
Reitano M. Counseling patients with Genital warts. Am J Med. 1997,102(5A):35-43
Turazza E, Lapena A, sprovieri O, et al. low-risk human papilloma virus type 6 and 11 associated with carcinomas of the genital and upper aero – digestive tract acta. Obstet Gynecol Scand. 1997, 79:271-276.
Palefsky JM, Holly EA, Ralston ML, et al. Anal cytological abnormalities and anal HPV infection in men with centers for disease control group IV HIV disease. Genitourin Med. 1997, 73:174-180.
Strehler E, sterzik K, Malthaner D, et al. Influence of ovarian stimulation on the detection of human papilloma virus DNA in cervical scrapes obtained from patients undergoing assisted reproductive techniques. Fertil Steril. 1999, 71(5): 815-20.
Motoyasu sugase, Toshihiko M. Distinct manifestations of Human papilloma viruses in the vagina. Int J Cancer. 1997, 412 - 415.
Volker A, Carlo, Mirka S, et al. Detection and Typing of Human papillomavirus in Biopsy and cytological specimens by polymerase chain reaction and restriction enzyme Analysis. J Med Viro. 1996, 48: 161 - 170.
Rezza G, Giuliani M, Branca M, et al. Determinats of squamous intraepithelial lesions (SIL) on pap smear the role of HPV infection and HIV-1-induced immuno suppresion dianaids collaborative study group. Eur J Epidemiol. 1997,13(8):937-43.
Kjaer SK, Van den Brule AJ, Bock JE, et al. Determinants for genital human papilloma virus (HPV) infection in 1000 randomly chosen young Danish with normal pap smear. Cancer Epidemiol Biomarkers Prev. 1997, 6(10):799-805.
Van Muyden RC, Ter Garnsek BW, Smedts FM, et al. Detection and typing of human papillomavirus in cervical carcinoma in Russian women a prognostic study. Cancer. 1999, 85(9):2011-6.
Cuzick J, Beverly E, Ho L,Tervy G, Mielzynska I, lorincz A. HPV testing in primary screening older women. Br J Cancer. 1999, 81(3):554-8.
Hwang T. Detection and typing of human papillomavirus DNA by PCR using consensus primers in various cervical lesions of korean women. J Korean Med Sci. 1999, 14(6):593-9.
Harnish DG, Velland LM, scheid EE,et. al. Evaluation of human papilloma virus-consensus for HPV detection by the polymerase chain reaction. Mol Cell Probes 1999, 13(1):9-21.
Konno R, Paez CL, Sato S, et al. HPV, histologic grade and age Risk factors for the progression of cervical intraepithelial neoplasia. J Repord Med. 1998,43(7): 561 - 6.
Journal of Reproduction & Infertility
Volume 1, Issue 3
Summer 2000
Pages 18-22