Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Morphological comparison of spermatozoa taken from TESE and PESA specimens in infertile men who referred to the infertility sections of Shariati Hospital and Navid Clinic in 1378

Authors
Hojjatallah Saeidi Saeidabady 1
Ashraf Alyasin 2
Marzieh Agha Hossinei 2
Afsaneh Khademi 2
Hamid Sahebkashaf 3
Saeed Sahebkashaf 3
Omid Poyan 2
1 IVF Laboratory, Shariati Hospital, Tehran Medical Sciences, University, Tehran, Iran
2 Department of Gynecology and Obstetrics ,Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3 Navid Fertility & Infertility Clinic, Tehran, Iran
Abstract
In this study morphology of testicular and epididymal sperm was assessed. Morphology of sperms was assessed by using Tygerbery strict criteria. TESE and PESA were done by conventional method under local anesthesia.Percentages of normal and abnormal sperm was studied. The percentage of normal testicular sperm ( 6.2% ) differed significantly from the percentage of normal epididymal sperm (18.6%). Percentages of abnormal form of head and tail differs in two groups but Percentage of midpiece abmormalities were not significantly different between the two groups.To assess the predictive value of Tygerberg strict criteria for determining morphology of testicular sperms we need a healthy control group.
Keywords
morphology
PESA
Sperm
Testicular sperm extraction
  1. Nagy Z, Liu J, Cilver S, et al. Using ejaculated fresh and frozen-thawed epididymal and testicular spermatozoa gives rise to comparable results after intracytoplasmic sperm injection. Fertil steril. 1995, 63:808-15.
  2. Schoysman R, Vanderzwalerman P, Nijs M, et al. Pregnancy after fertilization with human spermatozoa. Lancet. 1993, 342: 1237.
  3. Svalander P, Jakobsson AH, Forsberg A, et al. The outcome of intracytoplasmic sperm injection in unrelated to strict criteria sperm morphology. Hum Reprod. 1996, 11:1019-22.
  4. The Sperm Microaspiration Retrieval Techniques Study Group. Results in the United States with sperm microaspiration retrieval techniques and assisted reproductive technologies. J Urol. 1994, 151:1255-90.
  5. Bladou F, Grillo JM, Rossi D, et al.Epididymal sperm aspiration in conjunction with in vitro Fertilization and embryo transfer in cases of obstructive azoospermia. Hum Reprod. 1991, 9:1284-7.
  6. Kruger TF, Menkveld R, Stander FSH, et al. Sperm morphologic features as a prognostic in vitro fertilization. Fertil Steril. 1986, 46:1118-23.
  7. Graft I, Khalifa Y, Tsirigotis M, et al.Percutaneous epididymal sperm aspiration and intracytoplasmic sperm injection in the management of infertility due to obstructive azoospermia. Fertil Steril. 1995, 63:1038-42.
  8. Kruger TF, Acosta AA, Simmons K, et al.Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril. 1988, 49:112-7.
  9. Hall JA, Fishel SB, Timson J, et al. Human sperm morphology evaluation pre-and post Percoll gradient centrifugation. Hum Reprod. 1995, 10: 342-6.
  10. Steele EK, Meclure N, Lewis S, et al. A comparison of the morphology of testicular, epididymal and ejaculated sperm from fertile men and men with obstructive azoospermia.Fertil Steril. 2000, 6:1099-1103.
  11. Abuzeid MI, Sasy MA, Salem, et al.Testicular sperm extraction and intracytoplasmic sperm injection: a simplified method for treatment of obstructive azoospermia. Fertil Steril. 1997, 68:328-33 .
  12. Clermont Y. The cycle of the seminiferous epithelium in man. Am J Anat. 1963, 112:35-51.
  13. De Kretser DM. Ultrastructural features of spermiogenesis. Zell-forsch Mikrosk Anat.1969, 98:477-505.
Journal of Reproduction & Infertility
Volume 1, Issue 3
Summer 2000
Pages 55-60