Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Indoleamine 2,3-dioxygenase (IDO) is expressed at feto-placental unit throughout mouse gestation: An immunohistochemical study

Authors
Shayda Hemmati 1
Mahmood Jeddi-Tehrani 2
Ebrahim Torkabadi 3
Jamileh Ghasemi 3
Golnaz Ensieh Kazemi sefat 3
Parivash Danesh 4
Leila Barzegar Yarmohammadi 2
Mohammad Mehdi Akhondi 3
Amir Hassan Zarnani 5
1 Department of Cell and Molecular Biology, Khatam University, Tehran, Iran
2 Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
3 Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
4 Immunology Research Center, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5 Immunology Research Center, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Nanobiotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
Abstract
Introduction: The cells expressing Indoleamine 2, 3-dioxygenase (IDO) in feto-maternal interface mediate tryptophan catabolism, hence protect allogeneic fetus from lethal rejection by maternal immune responses. In this study, we report immuno-localization of IDO+ cells in murine reproductive tract and placenta throughout mouse pregnancy by immunohistochemistry.Materials and Methods: Syngeneic pregnant mice were examined for vaginal plug to discover about their state of pregnancy. A total of three pregnant mice were examined at each stage.The examination was further confirmed by the detection of sperm in vaginal smear. On the gestational days of 2nd, 12th and 18th, the uterus and oviduct were removed and expression of IDO was investigated in the endometrium, placenta and oviduct by immunohistochemistry. Results: Our results showed that IDO is expressed consistently in feto-maternal interface throughout pregnancy. In endometrium, expression of IDO was predomin-antly confined to luminal and glandular epithelial cells. Cells at junctional and labyrinth zones of placenta showed strong IDO immunoreactivity as well.Conclusion: Expression of IDO at the protein level in reproductive tract of pregnant mice during entire periods of gestation points to its potential protective role in maintenance of pregnancy. In our knowledge this is the first report of expression of IDO in feto-maternal phase during murine pregnancy.
Keywords
Decidua
Endometrium
immunohistochemistry
Indolamine 2,3- dioxygenase
placenta
Pregnancy
tolerance
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Journal of Reproduction & Infertility
Volume 10, Issue 3
Autumn 2009
Pages 177-183