Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Effects of Maternal Caffeine Consumption on Ovarian Follicle Development in Wistar Rats Offspring

Authors
Mehran Dorostghoal 1
Mahmood Khaksari Mahabadi 2
Sahar Adham 1
1 Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahwaz, Ahvaz, Iran
2 Departmant of Anatomy, Faculty of Veterinary, Shahid Chamran University of Ahwaz, Ahvaz, Iran
Abstract
Background: In recent years concerns have been raised about human reproductive disorders, specially the effects of environmental factors on human fertility and pregnancy outcome. Therefore, the present study was designed to assess the effects of maternal caffeine consumption on ovarian follicles development in rat offspring. Methods: 60 pregnant female rats were randomly divided into a control and two experimental groups. The rats in the two experimental groups received caffeine via drinking water during gestation (26 and 45 mg/kg) and lactation (25 and 35 mg/kg). The ovaries of the offspring were removed at 7, 14, 28, 60, 90 and 120 days after birth, and fixed in Bouin’s solution. By preparing serial tissue sections, structural changes in ovarian follicles and corpora lutea were studied during postnatal development. Results: The weight of ovaries decreased significantly (p < 0.05) in the high dose caffeine-treated group at all stages of postnatal development. Significant (p < 0.05) decreases were seen in the number of primordial follicles from day 7 to 120 after birth in the high dose caffeine-treated group. Moreover, the number of primary and secondary follicles decreased significantly on days 7, 14 and 28 as did the number of antral follicles on days 14 and 28 after birth (p < 0.05) in the high dose caffeine-treated group. The diameter of secondary and antral follicles decreased significantly (p < 0.05) in high dose caffeine-treated group on the early days of postnatal development. No statistically significant differences were seen in the number of corpora lutea between the groups. Conclusion: The present study shows that caffeine consumption during gestation and lactation affects the early stages of ovarian follicle development and reduces reproductive efficiency in the offspring of Wistar rats.
Keywords
Caffeine
Fertility
Ovarian follicles
Postnatal development
  1. Hruska KS, Furth PA, Seifer DB, Sharara FI, Flaws JA. Environmental factors in infertility. Clin Obstet Gynecol. 2000;43(4):821-9.
  2. Kumar S, Kumari A, Murarka S. Lifestyle factors in deteriorating male reproductive health. Indian J Exp Biol. 2009;47(8):615-24.
  3. Al-Shoshan AA. Predictors of caffeine consumption among young women. Pak J Nutr. 2007;6(6):597-602.
  4. Monroe J. Caffeine’s hook. Curr Health. 1998;2:5-12.
  5. Barone JJ, Roberts HR. Caffeine consumption. Food Chem Toxicol. 1996;34(1):119-29.
  6. Otberg N, Teichmann A, Rasuljev U, Sinkgraven R, Sterry W, Lademann J. Follicular penetration of topically applied caffeine via a shampoo formulation. Skin Pharmacol Physiol. 2007;20(4):195-8.
  7. Golding J. Reproduction and caffeine consumption--a literature review. Early Hum Dev. 1995;43(1):1-14.
  8. Aranda JV, Collinge JM, Zinman R, Watters G. Maturation of caffeine elimination in infancy. Arch Dis Child. 1979;54(12):946-9.
  9. Ramlau-Hansen CH, Thulstrup AM, Bonde JP, Olsen J, Bech BH. Semen quality according to prenatal coffee and present caffeine exposure: two decades of follow-up of a pregnancy cohort. Hum Reprod. 2008;23(12):2799-805.
  10. Nolen GA. The effect of brewed and instant coffee on reproduction and teratogenesis in the rat. Toxicol Appl Pharmacol. 1981;58(2):171-83.
  11. Wilcox A, Weinberg C, Baird D. Caffeinated beverages and decreased fertility. Lancet. 1988;2 (8626-8627):1453-6.
  12. Jagiello G, Ducayen M, Lin JS. Meiosis suppression by caffeine in female mice. Mol Gen Genet. 1972;118(3):209-14.
  13. Boyd EM, Dolman M, Knight LM, Sheppard EP. The chronic oral toxicity of caffeine. Can J Physiol Pharmacol. 1965;43(6):995-1007.
  14. Kren R, Ogushi S, Miyano T. Effect of caffeine on meiotic maturation of porcine oocytes. Zygote. 2004;12(1):31-8.
  15. Olsen J. Cigarette smoking, tea and coffee drinking, and subfecundity. Am J Epidemiol. 1991;133 (7):734-9.
  16. Alderete E, Eskenazi B, Sholtz R. Effect of cigarette smoking and coffee drinking on time to conception. Epidemiology. 1995;6(4):403-8.
  17. Caan B, Quesenberry CP Jr, Coates AO. Differences in fertility associated with caffeinated beverage consumption. Am J Public Health. 1998;88(2): 270-4.
  18. Pollard I, Williamson S, Magre S. Influence of caffeine administered during pregnancy on the early differentiation of fetal rat ovaries and testes. J Dev Physiol. 1990;13(2):59-65.
  19. Maalouf W, Lee JH, Campbell KH. Effects of caffeine on the developmental potential of in vitro matured, aged and denuded ovine oocytes. Hum Fertil. 2005;8(2):129-30.
  20. Grosso LM, Bracken MB. Caffeine metabolism, genetics, and perinatal outcomes: a review of exposure assessment considerations during pregnancy. Ann Epidemiol. 2005;15(6):460-6.
  21. Moore RW, Rudy TA, Lin TM, Ko K, Peterson RE. Abnormalities of sexual development in male rats with in utero and lactational exposure to the antiandrogenic plasticizer Di(2-ethylhexyl) phthalate. Environ Health Perspect. 2001;109(3):229-37.
  22. Odum J, Lefevre PA, Tinwell H, Van Miller JP, Joiner RL, Chapin RE, et al. Comparison of the developmental and reproductive toxicity of diethylstilbestrol administered to rats in utero, lactationally, preweaning, or postweaning. Toxicol Sci. 2002; 68(1):147-63.
  23. Hughes RN, Beveridge IJ. Behavioral effects of exposure to caffeine during gestation, lactation or both. Neurotoxicol Teratol. 1991;13(6):641-7.
  24. Hughes RN, Loader VG. Effects on elevated plusmaze behavior of exposure to caffeine during both gestation and lactation. Psychobiology. 1996;24 (4):314-9.
  25. Bolon B, Bucci TJ, Warbritton AR, Chen JJ, Mat-tison DR, Heindel JJ. Differential follicle counts as a screen for chemically induced ovarian toxicity in mice: results from continuous breeding bioassays. Fundam Appl Toxicol. 1997;39(1):1-10.
  26. Britt KL, Drummond AE, Cox VA, Dyson M, Wreford NG, Jones ME, et al. An age-related ovarian phenotype in mice with targeted disruption of the Cyp 19 (aromatase) gene. Endocrinology. 2000;141(7):2614-23.
  27. Myers M, Britt KL, Wreford NG, Ebling FJ, Kerr JB. Methods for quantifying follicular numbers within the mouse ovary. Reproduction. 2004;127 (5):569-80.
  28. McGee EA, Hsueh AJ. Initial and cyclic recruitment of ovarian follicles. Endocr Rev. 2000;21(2): 200-14.
  29. Hoyer PB. Damage to ovarian development and function. Cell Tissue Res. 2005;322(1):99-106.
  30. Guigon CJ, Magre S. Contribution of germ cells to the differentiation and maturation of the ovary: insights from models of germ cell depletion. Biol Reprod. 2006;74(3):450-8.
  31. Kezele P, Skinner MK. Regulation of ovarian primordial follicle assembly and development by estrogen and progesterone: endocrine model of follicle assembly. Endocrinology. 2003;144(8): 3329-37.
  32. Hirshfield AN. Development of follicles in the mammalian ovary. Int Rev Cytol. 1991;124:43-101.
  33. Rennels EG. Influence of hormones on the histochemistry of ovarian interstitial tissue in the immature rat. Am J Anat. 1951;88(1):63-107.
  34. Hoyer PB, Sipes IG. Assessment of follicle destruction in chemical-induced ovarian toxicity. Annu Rev Pharmacol Toxicol. 1996;36:307-31.
  35. Hooser SB, Douds DP, DeMerell DG, Hoyer PB, Sipes IG. Long-term ovarian and gonadotropin changes in mice exposed to 4-vinylcyclohexene. Reprod Toxicol. 1994;8(4):315-23.
  36. Generoso WM, Stout SK, Huff SW. Effects of alkylating chemicals on reproductive capacity of adult female mice. Mutat Res. 1971;13(2):172-84.
  37. Axelrod J, Reichenthal J. The fate of caffeine in man and a method for its estimation in biological material. J Pharmacol Exp Ther. 1953;107(4):519-23.
  38. Müller WU, Bauch T, Wojcik A, Böcker W, Streffer C. Comet assay studies indicate that caffeinemediated increase in radiation risk of embryos is due to inhibition of DNA repair. Mutagenesis. 1996;11(1):57-60.
Journal of Reproduction & Infertility
Volume 12, Issue 1
Winter 2011
Pages 15-22