Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Effects of Nicotine and Ethanol Administration on the Seminal Vesicle of Adult Rats

Authors
Mohsen Basiri 1
Masoud Ezat Abadipour 2
Vahid Hemayatkhah Djahromi 3
Naghmeh Shahidi Zandi 3
Arash Sarv Azad 4
Seyed Noureddin Nematollahi-Mahani 5
1 Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran
2 Professor, Anatomy Department, Faculty of Medicine, Kerman Medical Sciences University, Kerman, Iran
3 Department of Biology, Faculty of Islamic Azad University, Jahrom Branch, Jahrom, Iran
4 Department of Anatomy, Faculty of Medicine, Tehran Medical Sciences University, Tehran, Iran
5 Department of Anatomy, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Abstract
Background: The effects of cigarette and alcohol on male reproductive system have been studied mainly on the testis and prostate but studies on their co-administration on the seminal vesicle which produces about 70% of the semen volume are scarce. Therefore, the present study evaluated the effects of nicotine and/ or alcohol on the glandular epithelial cells of seminal vesicle in adult rats. Methods: In this study, 50 adult Wistar rats, aged 9 weeks, were randomly divided into five groups, including: sham, control (0.09% normal saline), 20% ethanol (2 ml/kg), nicotine (0.1 mg/kg) and ethanol-nicotine. Ethanol was given via oral gavage but nicotine was administered subcutaneously for 50 days. Blood samples were collected prior to intracardiac perfusion. The seminal vesicles were later dissected and tissue samples were stained by H&E for morphologic and morphometric studies. One-way ANOVA and Tukey’s post-hoc test were used for data analysis. A p-value
Keywords
cigarette
Ethanol
Nicotine
seminal vesicle
testosterone
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Journal of Reproduction & Infertility
Volume 12, Issue 2
Spring 2011
Pages 109-115