Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Effects of Nicotine on Sperm Characteristics and Fertility Profile in Adult Male Rats: A Possible

Authors
Ibukun Peter Oyeyipo 1
Raji Yinusa 2
Benjamin Obukowho Emikpe 3
Adeyombo Folashade Bolarinwa 2
1 Department of Physiology, College of Health Sciences, Osun State University, Osun State, Nigeria
2 Department of Physiology, College of Medicine, University of Ibadan, Oyo State, Nigeria
3 Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Oyo State, Nigeria
Abstract
Background: Infertility is common among couples of child-bearing age and ap-proximately half of known causes of primary infertility are attributable to male factor. It is still unclear whether the injurious effects of cigarette smoking on sperm characteristics and infertility are due to nicotine. Therefore, the present study investigated the effects of orally administered of nicotine on sperm characteristics and libido in adult male albino rats. The study also sought nicotine effects on fertility rate, litter size and weight in female animals cohabited with nicotine treated male rats. Methods: Forty male and twenty-five female rats were used for the study. The male rats were divided into five groups and were treated for a period of 30 days with nicotine 0.5 mg/kg (low dose) and 1.0 mg/kg (high dose) per body weight while the control rats received 0.2 ml/kg normal saline. The fourth and fifth groups were gavaged with 0.5 mg/kg and 1.0 mg/kg body weight of nicotine but were left untreated for another 30 days. These groups served as the recovery groups. At the end of each experimental period, sperm analysis, fertility study, litter weight and size were determined. Results: Sperm motility and count significantly decreased (P < 0.05) while the percentage of abnormality significantly increased (P < 0.05) in both treatment groups. However, there was an insignificant decrease (P > 0.05) in the viability and semen volume of the treated groups. Fertility studies revealed that nicotine reduced libido in male rats, litter weight and number delivered by the untreated female during the experiments. Conclusion: The present study showed that nicotine has a dose-dependent deleterious effect on the sperm characteristics and that fertility is ameliorated by nicotine cessation in male rats.
Keywords
Hypertension
Pregnancy
risk factor
Yales CA, Thomas C, Kovacs GT, De Krester DM. Androgen, male factor, infertility and IVF. In: Wood C, Trouson A, editors. Clinical in vitro fertilization. USA: Springer-Verlag; 1989. pp. 95-111.
Yeşilli C, Mungan G, Seçkiner I, Akduman B, Açikgöz S, Altan K, et al. Effect of varicocelectomy on sperm creatine kinase, HspA2 chaperone protein (creatine kinase-M type), LDH, LDH-X, and lipid peroxidation product levels in infertile men with varicocele. Urology. 2005;66(3):610-5.
Reijo R, Alagappan RK, Patrizio P, Page DC. Severe oligozoospermia resulting from deletions of azoospermia factor gene on Y chromosome. Lancet. 1996;347(9011):1290-3.
Bonde JP. Male fertility. In: Comhaire FM, editor. Chapman and Hall Medicals. New York: Chapman and Hall; 1996. p. 266-84.
Kapoor D, Jones TH. Smoking and hormones in health and endocrine disorders. Eur J Endocrinol. 2005;152(4):491-9.
Russell MA, Jarvis MJ, Devitt G, Feyerabend C. Nicotine intake by snuff users. Br Med J (Clin Res Ed). 1981;283(6295):814-7.
Armitage AK, Dollery CT, George CF, Houseman TH, Lewis PJ, Turner DM. Absorption and metabolism of nicotine from cigarettes. Br Med J. 1975;4 (5992):313-6.
Weisberg E. Smoking and reproductive health. Clin Reprod Fertil. 1985;3(3):175-86.
Aydos K, Güven MC, Can B, Ergün A. Nicotine toxicity to the ultrastructure of the testis in rats. BJU Int. 2001;88(6):622-6.
Mlynarcikova A, Fickova M, Scsukova S. Ovarian intrafollicular processes as a target for cigarette smoke components and selected environmental reproductive disruptors. Endocr Regul. 2005;39(1): 21-32.
Tankó LB, Christiansen C. An update on the antiestrogenic effect of smoking: a literature review with implications for researchers and practitioners. Menopause. 2004;11(1):104-9.
Jones TH, Jones RD, Channer KS. Testosterone and its beneficial actions on cardiovascular disorders. In: Plotsky PM, Hunzicke-Dunne M, Rose JC, Brenner RM, editors. Recent research developments in endocrinology & metabolism, Volume 1. USA: Transworld research network; 2003. p. 143-68.
Carmines EL, Gaworski CL, Faqi AS, Rajendran N. In utero exposure to 1R4F reference cigarette smoke: evaluation of developmental toxicity. Toxicol Sci. 2003;75(1):134-47.
Gaworski CL, Carmines EL, Faqi AS, Rajendran N. In utero and lactation exposure of rats to 1R4F reference cigarette mainstream smoke: effect on prenatal and postnatal development. Toxicol Sci. 2004;79(1):157-69.
Oyeyipo IP, Raji Y, Emikpe BO, Bolarinwa AF. Effects of oral administration of nicotine on organ weight, serum testosterone level and testicular pathology in adult male rats. Niger J Psychol Sci. 2010;25(1):81-86.
Iranloye BO, Bolarinwa AF. Effect of nicotine administration on weight and histology of some vital visceral organs in female albino rats. Niger J Physiol Sci. 2009;24(1):7-12.
Dhawan K, Sharma A. Prevention of chronic alcohol and nicotine-induced azospermia, sterility and decreased libido, by a novel tri-substituted benzo-flavone moiety from Passiflora incarnata Linneaus in healthy male rats. Life Sci. 2002;71(26):3059-69.
Bolarinwa Y. Gastric acid secretion in pregnant and lactating rat. Trop Vet.1993;11:35-38.
Raji Y, Oloyo AK, Morakinyo AO. Effect of methanol extract of Ricinus communis seed on reproduction of male rats. Asian J Androl. 2006;8 (1):115-21.
Raji Y, Udoh US, Mewoyeka OO, Ononye FC, Bolarinwa AF. Implication of reproductive endocrine malfunction in male antifertility efficacy of Azadirachta indica extract in rats. Afr J Med Med Sci. 2003;32(2):159-65.
Morrissey RE, Schwetz BA, Lamb JC 4th, Ross MD, Teague JL, Morris RW. Evaluation of rodent sperm, vaginal cytology, and reproductive organ weight data from National Toxicology Program 13-week studies. Fundam Appl Toxicol. 1988;11 (2):343-58.
Wyrobek AJ, Bruce WR. The induction of sperm shape abnormalities in mice and humans. In: Hollaender A, De Serres FJ. Chemical mutagens Vol. 5. New York: Plenum Press; 1980. p. 257-85.
Freund M, Carol B. Factors affecting haemocytometer count of sperm concentration in human semen. J Reprod Fertil. 1964;8:149-55.
Andrzej B, Hahn DW, Foldsey RG, Megure JL. Experimental studies in the development of male contraceptives. In: Zatuchni G, Goldsmith A, Spieller JM, Saara JJ, editors. Male contraception, advances and future prospects. New York: Harper and Row; 1985. p. 158-82.
Pacifici R, Altieri I, Gandini L, Lenzi A, Passa AR, Pichini S, et al. Environmental tobacco smoke: nicotine and cotinine concentration in semen. Environ Res. 1995;68(1):69-72.
Sharpe RM, Maddocks S, Millar M, Kerr JB, Saunders PT, McKinnell C. Testosterone and spermatogenesis. Identification of stage-specific, androgen-regulated proteins secreted by adult rat seminiferous tubules. J Androl. 1992;13(2):172-84.
Tulsiani DR, Orgebin-Crist MC, Skudlarek MD. Role of luminal fluid glycosyltransferases and glycosidases in the modification of rat sperm plasma membrane glycoproteins during epididymal maturation. J Reprod Fertil Suppl. 1998;53:85-97.
Mooradian AD, Morley JE, Korenman SG. Biological actions of androgens. Endocr Rev. 1987;8 (1):1-28.
Yamamoto Y, Isoyama E, Sofikitis N, Miyagawa I. Effects of smoking on testicular function and fertilizing potential in rats. Urol Res. 1998;26(1):45-8.
Journal of Reproduction & Infertility
Volume 12, Issue 3
Summer 2011
Pages 201-207