Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Effects of Vitrification on Immature and in vitro Matured, Denuded and Cumulus Compact Goat Oocytes and their Subsequent Fertilization

Authors
Govind Narayan Purohit
Harikesh Meena
Kanika Solanki
Department of Veterinary Gynecology and Obstetrics, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Rajasthan, India
Abstract
Background: Vitrification has proven to be more effective than slow freezing methods to cryopreserve mammalian oocytes. The objectives of this study were to evaluate the effects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization. Methods: Oocytes were either cryopreserved as immature cumulus compact (IMCC) (n=98 Exp 1; 102 Exp 2) and immature denuded (IMDN) (n=127 Exp 1; 109 Exp 2) or were first matured in vitro for 28 h and then cryopreserved as mature cumulus compact (MCC) (n=109 Exp 1; 89 Exp 2) or mature denuded (MDN) (n=112 Exp 1; 110 Exp 2) oocytes in four groups. The vitrification solution comprised of Dulbecco’s phosphate buffered saline supplemented with 0.5% sucrose, 0.4% bovine serum albumin and 8 M propylene glycol. After 7 days of cryopreservation in liquid nitrogen, oocytes in all groups were evaluated for normal morphologic survival and in vitro maturation (Experiment 1) and fertilization in vitro using epididymal buck spermatozoa (Experiment 2). Results: The number of oocytes retaining normal morphology was significantly higher (p
Keywords
In vitro fertilization
Goat
In vitro Maturation
Oocytes
Vitrification
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Journal of Reproduction & Infertility
Volume 13, Issue 1
Winter 2012
Pages 53-59