Effects of Vitrification on Immature and in vitro Matured, Denuded and Cumulus Compact Goat Oocytes and their Subsequent Fertilization

Purohit, Govind Narayan; Meena, Harikesh; Solanki, Kanika

Effects of Vitrification on Immature and in vitro Matured, Denuded and Cumulus Compact Goat Oocytes and their Subsequent Fertilization

Authors

Govind Narayan Purohit

Harikesh Meena

Kanika Solanki

Department of Veterinary Gynecology and Obstetrics, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Rajasthan, India

Abstract

Background: Vitrification has proven to be more effective than slow freezing methods to cryopreserve mammalian oocytes. The objectives of this study were to evaluate the effects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization. Methods: Oocytes were either cryopreserved as immature cumulus compact (IMCC) (n=98 Exp 1; 102 Exp 2) and immature denuded (IMDN) (n=127 Exp 1; 109 Exp 2) or were first matured in vitro for 28 h and then cryopreserved as mature cumulus compact (MCC) (n=109 Exp 1; 89 Exp 2) or mature denuded (MDN) (n=112 Exp 1; 110 Exp 2) oocytes in four groups. The vitrification solution comprised of Dulbecco’s phosphate buffered saline supplemented with 0.5% sucrose, 0.4% bovine serum albumin and 8 M propylene glycol. After 7 days of cryopreservation in liquid nitrogen, oocytes in all groups were evaluated for normal morphologic survival and in vitro maturation (Experiment 1) and fertilization in vitro using epididymal buck spermatozoa (Experiment 2). Results: The number of oocytes retaining normal morphology was significantly higher (p

Keywords

In vitro fertilization

Goat

In vitro Maturation

Oocytes

Vitrification

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