Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Correlation of Sperm Associated Antigen 11 (SPAG11) and its Isoforms with Varicocele in Rats

Authors
Hong Tian 1
Yong-Wei Huo 1
Jin-Song Zhou 1
Li-Rong Wang 1
Qiu-Yang Zhang 2
Shu-Dong Qiu 1
1 Department of Anatomy, Histology and Embryology, School of Medicine, Xi’an Jiaotong University, Xi’an, China; Research Center of Reproductive Medicine, School of Medicine, Xi’an Jiaotong University, Xi’an, China
2 Research Center of Reproductive Medicine, School of Medicine, Xi’an Jiaotong University, Xi’an, China
Abstract
Background: We undertook this study to investigate the variation relationship of sperm associated antigen 11 (Spag11) mRNA expression and SPAG11E protein in the epididymis and spermatozoa of experimental left varicocele (ELV) rats. These findings could contribute to the understanding of the role of epididymal proteins in sperm functions and the mechanism of male infertility induced by varicocele. Methods: The ELV model was established in adolescent male Sprague-Dawley rats. Four weeks after the operation, tissue distribution and changes in the expressions of Spag11 mRNA and SPAG11E protein caused by ELV in the whole of left epididymis and spermatozoa were studied using quantitative reverse transcription-polymer-ase chain reaction (RT-QPCR), immunohistochemistry and immunofluorescence. Significant differences were identified using one-way ANOVA followed by Student-Newman-Keuls test. Significance level (p) was fixed at 0.05. Results: The expected product of Spag11 was 96 bp that amplified by RT-QPCR was detected in the epididymal tissue and spermatozoa. SPAG11E protein was confined mainly to the supranuclear region of the principal cells and the stereocilium of the epididymal epithelium, it was concentrated on the acrosome and the tail of spermatozoa except the terminal piece. Statistical analyses of the images and the data indicated that Spag11 mRNA and SPAG11E protein expressions in the left epididymis and spermatozoa of ELV rats presented a considerable decrease (p<0.001) compared with that of the corresponding control group. Conclusion: The expressions of Spag11 mRNA and SPAG11E protein declined markedly in ELV rats, which suggest that SPAG11E may not only play an important role in sperm maturation, but it may also be influenced by varicocele.
Keywords
epididymis
Rat
Sperm associated antigen 11 protein
Spermatozoa
Varicocele
Yenugu S, Hamil KG, Grossman G, Petrusz P, French FS, Hall SH. Identification, cloning and functional characterization of novel sperm associated antigen 11 (SPAG11) isoforms in the rat. Reprod Biol Endocrinol. 2006;4:23.
von Horsten HH, Derr P, Kirchhoff C. Novel antimicrobial peptide of human epididymal duct origin. Biol Reprod. 2002;67(3):804-13.
Fröhlich O, Po C, Young LG. Organization of the human gene encoding the epididymis-specific EP2 protein variants and its relationship to defensin genes. Biol Reprod. 2001;64(4):1072-9.
Fröhlich O, Ibrahim NM, Young LG. EP2 splicing variants in rhesus monkey (Macaca mulatta) epididymis. Biol Reprod. 2003;69(1):294-300.
Avellar MC, Honda L, Hamil KG, Yenugu S, Grossman G, Petrusz P, et al. Differential expression and antibacterial activity of epididymis protein 2 isoforms in the male reproductive tract of human and rhesus monkey (Macaca mulatta). Biol Reprod. 2004;71(5):1453-60.
Fröhlich O, Po C, Murphy T, Young LG. Multiple promoter and splicing mRNA variants of the epididymis-specific gene EP2. J Androl. 2000;21(3):421-30.
Hall SH, Yenugu S, Radhakrishnan Y, Avellar MC, Petrusz P, French FS. Characterization and functions of beta defensins in the epididymis. Asian J Androl. 2007;9(4):453-62.
Li P, Chan HC, He B, So SC, Chung YW, Shang Q, et al. An antimicrobial peptide gene found in the male reproductive system of rats. Science. 2001;291(5509):1783-5.
Zhou CX, Zhang YL, Xiao L, Zheng M, Leung KM, Chan MY, et al. An epididymis-specific beta-defensin is important for the initiation of sperm maturation. Nat Cell Biol. 2004;6(5):458-64.
Tian H, Qiu SD, Zhang QY, Xue X, Ge L, Wang LR. [Effects of experimental left varicocele on SPAG11 mRNA and SPAG11E in the testis and epididymis of adolescent rats]. Zhonghua Nan Ke Xue. 2008;14(3):200-5. Chinese.
Hamil KG, Sivashanmugam P, Richardson RT, Grossman G, Ruben SM, Mohler JL, et al. HE2 beta and HE2gamma, new members of an epididymis-specific family of androgen-regulated proteins in the human. Endocrinology. 2000;141(3):1245-53.
Rodríguez CM, Kirby JL, Hinton BT. Regulation of gene transcription in the epididymis. Reproduction. 2001;122(1):41-8.
Ibrahim NM, Young LG, Fröhlich O. Epididymal specificity and androgen regulation of rat EP2. Biol Reprod. 2001;65(2):575-80.
Yenugu S, Hamil KG, French FS, Hall SH. Antimicrobial actions of human and macaque sperm associated antigen (SPAG) 11 isoforms: influence of the N-terminal peptide. Mol Cell Biochem. 2006;284(1-2):25-37.
Zhang QY, Qiu SD, Ma XN, Yu HM, Wu YW. Effect of experimental varicocele on structure and function of epididymis in adolescent rats. Asian J Androl. 2003;5(2):108-12.
Ozturk U, Kefeli M, Asci R, Akpolat I, Buyukalpelli R, Sarikaya S. The effects of experimental left varicocele on the epididymis. Syst Biol Reprod Med. 2008;54(4-5):177-84.
Journal of Reproduction & Infertility
Volume 13, Issue 4
Autumn 2012
Pages 241-247