Classical and Molecular Methods for Evaluation of Chlamydia trachomatis Infection in Women with Tubal Factor Infertility

Hajikhani, Bahareh; Motallebi, Tayebeh; Norouzi, Jamileh; Bahador, Abbas; Bagheri, Rezvan; Asgari, Soheila; Chamani Tabriz, Leili

Classical and Molecular Methods for Evaluation of Chlamydia trachomatis Infection in Women with Tubal Factor Infertility

Authors

Bahareh Hajikhani ¹

Tayebeh Motallebi ²

Jamileh Norouzi ²

Abbas Bahador ³

Rezvan Bagheri ¹

Soheila Asgari ⁴

Leili Chamani Tabriz ¹

¹ Reproductive Infections Department of Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

² Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran

³ Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

⁴ International Campus, Tehran University of Medical Sciences, Kish Island, Iran

Abstract

Background: Chlamydia trachomatis is the most reported bacterial sexually transmitted disease, especially among young women worldwide. aim of this study was comparison to evaluate the prevalence of Chlamydia trachomatis infection in woman with tubal infertility by means of PCR and cell culture techniques. Methods: Fifty-one women with confirmed TFI were enrolled in this study in (avicenna infertility Clinic) between January 2010 and January 2011. Cervical swab and cytobrush specimens were collected from each patient by gynecologists and sent to laboratory in transport media. Detection of Chlamydia trachomatis in samples was performed using PCR and bacteria culture in MacCoy cell line. The data were analyzed by Fisher’s exact test and independent t-test. Statistical significance was established at a p-value

Keywords

cell culture

Chlamydia trachomatis

Cytobrush

PCR

Swab

Beagley KW, Timms P. Chlamydia trachomatis infection: incidence, health costs and prospects for vaccine development. J Reprod Immunol. 2000;48 (1):47-68.

Patel AL, Sachdev D, Nagpal P, Chaudhry U, Son-kar SC, Mendiratta SL, et al. Prevalence of Chlamydia infection among women visiting a gynaecology outpatient department: evaluation of an in-house PCR assay for detection of Chlamydia trachomatis. Ann Clin Microbiol Antimicrob. 2010; 9:24.

Morré SA, Rozendaal L, van Valkengoed IG, Boe-ke AJ, van Voorst Vader PC, Schirm J, et al. Urogenital Chlamydia trachomatis serovars in men and women with a symptomatic or asymptomatic infection: an association with clinical manifestations? J Clin Microbiol. 2000;38(6): 2292-6.

Cetin MT, Vardar MA, Aridogan N, Köksal F, Kiliç B, Burgut R. Role of Chlamydia trachomatis infections in infertility due to tubal factor. Indian J Med Res. 1992;95:139-43.

Murawski M, Matusiak M, Gryboś M. [Chlamydia trachomatis as an etiological factor of marital infertility--is a routine diagnostics worth to perform?]. Wiad Lek. 2007;60(9-10):445-8. Polish.

Watson EJ, Templeton A, Russell I, Paavonen J, Mardh PA, Stary A, et al. The accuracy and efficacy of screening tests for Chlamydia trachomatis: a systematic review. J Med Microbiol. 2002;51(12): 1021-31.