Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Classical and Molecular Methods for Evaluation of Chlamydia trachomatis Infection in Women with Tubal Factor Infertility

Authors
Bahareh Hajikhani 1
Tayebeh Motallebi 2
Jamileh Norouzi 2
Abbas Bahador 3
Rezvan Bagheri 1
Soheila Asgari 4
Leili Chamani Tabriz 1
1 Reproductive Infections Department of Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
2 Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran
3 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4 International Campus, Tehran University of Medical Sciences, Kish Island, Iran
Abstract
Background: Chlamydia trachomatis is the most reported bacterial sexually transmitted disease, especially among young women worldwide. aim of this study was comparison to evaluate the prevalence of Chlamydia trachomatis infection in woman with tubal infertility by means of PCR and cell culture techniques. Methods: Fifty-one women with confirmed TFI were enrolled in this study in (avicenna infertility Clinic) between January 2010 and January 2011. Cervical swab and cytobrush specimens were collected from each patient by gynecologists and sent to laboratory in transport media. Detection of Chlamydia trachomatis in samples was performed using PCR and bacteria culture in MacCoy cell line. The data were analyzed by Fisher’s exact test and independent t-test. Statistical significance was established at a p-value
Keywords
cell culture
Chlamydia trachomatis
Cytobrush
PCR
Swab
Journal of Reproduction & Infertility
Volume 14, Issue 1
Winter 2013
Pages 29-33