Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Assisted Reproductive Technology after the Birth of Louise Brown

Author
Department of Obstetrics and Gynaecology, Faculty of Medicine, Dammam University, Dammam, Saudi Arabia
Abstract
Background: Public interest in Assisted Reproductive Technology (ART) has remained high since the birth of the world’s first in vitro fertilization baby, Louise Brown, in the United Kingdom. ART allows scientists to manipulate the fertilization process in order to bypass some pathological obstacles such as blocked fallopian tubes and non-functioning ovaries in the females, and blocked vas deferens and low sperm count in the males. The purpose was to provide a historical outline and identify the researches that most contributed to ART. Methods: A review of published experimental and clinical studies of assisted reproduction carried out at the University of Bristol library website (MetaLib®). A cross-search of seven different medical databases (AMED-Allied and Complementary Medicine Database, BIOSIS Previews on Web of Knowledge, Cochrane Library, Embase, and the Medline on Web of Knowledge, OvidSP and PubMed) was completed by using the key words to explore the major milestones and progress in the development and implementation of ART. Results: A speedy advancement in the development of different assisted reproductive techniques makes infertility problem more treatable than it ever had been. Conclusion: Although no other field in the medicine has integrated new knowledge into the daily practice more quickly than ART yet, there is a need for social research to counterbalance the dominance of biomedical one, in particular the people’s actual experiences and expectations of ART.
Keywords

  1. De Kretzer D, Dennis P, Hudson B, Leeton J, Lopata A, Outch K, et al. Transfer of a human zygote. Lancet. 1973;2(7831):728-9.
  2. Steptoe PC, Edwards RG. Reimplantation of a human embryo with subsequent tubal pregnancy. Lancet.1976;1(7965):880-2.
  3. Steptoe PC, Edwards RG. Birth after the reimplantation of a human embryo. Lancet.1978;2(8085):366.
  4. Edwards RG. An astonishing journey into reproductive genetics since the 1950's. Reprod Nutr Dev. 2005;45(3):299-306.
  5. Lopata A, Johnston IW, Hoult IJ, Speirs AI. Pregnancy following intrauterine implantation of an embryo obtained by in vitro fertilization of a preovulatory egg. Fertil Steril. 1980;33(2):117-20.
  6. Cohen J, Trounson A, Dawson K, Jones H, Hazekamp J, Nygren KG, Hamberger L. The early days of IVF outside the UK. Hum Reprod Update. 2005;11(5):439-59. Epub 2005 May 27.
  7. Lopata A, Johnston IW, Hoult IJ, Speirs AI. Pregnancy following intrauterine implantation of an embryo obtained by in vitro fertilization of a preovu-latory egg. Fertil Steril. 1980;33(2):117-20.
  8. Al-Nuaim L, Jenkins J. A brief historical overview of assisted reproduction. South Afr J Obstet Gynecol. 2007;13(2):38-41.
  9. Trounson AO, Leeton JF, Wood C, Webb J, Wood J. Pregnancies in humans by fertilization in vitro and embryo transfer in the controlled ovulatory cycle. Science. 1981;212(4495):681-2.
  10. Trounson AO, Mohr LR, Wood C, Leeton JF. Effect of delayed insemination on in-vitro fertilization, culture and transfer of human embryos. J Reprod Fertil. 1982;64(2):285-94.
  11. Sathananthan AH, Trounson AO. Ultrastructural observations on cortical granules in human follicular oocytes cultured in vitro. Gamete Res. 1982;5:191-8.
  12. Testart J, Frydman R, Feinstein MC, Thebault A, Roger M, Scholler R. Interpretation of plasma luteinizing hormone assay for the collection of mature oocytes from women: definition of a luteinizing hormone surge-initiating rise. Fertil Steril. 1981;36 (1):50-4.
  13. Hamberger L, Wikland M, Nilsson L, Janson PO, Sjogren A, Hillensjo T. Methods for aspiration of human oocytes by various techniques. Acta Med Rom. 1982;20:370-8
  14. Mohr LR, Trounson AO. The use of fluorescein diacetate to assess embryo viability in the mouse. J Reprod Fertil. 1980;58(1):189-96.
  15. Fleming R, Adam AH, Barlow DH, Black WP, MacNaughton MC, Coutts JR. A new systematic treatment for infertile women with abnormal hormone profiles. Br J Obstet Gynaecol. 1982;89(1):80-3.
  16. Lenz S, Lauritsen JG. Ultrasonically guided percutaneous aspiration of human follicles under local anesthesia: a new method of collecting oocytes for in vitro fertilization. Fertil Steril. 1982;38(6):673-7.
  17. Trounson A, Leeton J, Besanko M, Wood C, Conti A. Pregnancy established in an infertile patient after transfer of a donated embryo fertilised in vitro. Br Med J (Clin Res Ed). 1983;286(6368):835-8.
  18. Trounson A, Mohr L. Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature. 1983;305(5936):707-9.
  19. Veeck LL, Wortham JW Jr, Witmyer J, Sandow BA, Acosta AA, Garcia JE, et al. Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril. 1983;39(5):594-602.
  20. Gleicher N, Friberg J, Fullan N, Giglia RV, Mayden K, Kesky T, et al. EGG retrieval for in vitro fertilisation by sonographically controlled vaginal culdocentesis. Lancet. 1983;2(8348):508-9.
  21. Casper RF, Wilson E, Collins JA, Brown SF, Parker JA. Enhancement of human implantation by exogenous chorionic gonadotropin. Lancet. 1983;2(8360):1191.
  22. Victoria Government. Infertility (Medical Procedures) Act 1984. Published by authority of Victoria Government Gazette. 1985;10163:47.
  23. Asch RH, Ellsworth LR, Balmaceda JP, Wong PC. Pregnancy after translaparoscopic gamete intrafal-lopian transfer. Lancet. 1984;2(8410):1034-5.
  24. Lutjen P, Trounson A, Leeton J, Findlay J, Wood C, Renou P. The establishment and maintenance of pregnancy using in vitro fertilization and embryo donation in a patient with primary ovarian failure. Nature. 1984;307(5947):174-5.
  25. Schulman JD, Dorfmann AD, Jones SL, Pitt CC, Joyce B, Patton LA. Outpatient in vitro fertilization using transvaginal ultrasound-guided oocyte retrieval. Obstet Gynecol. 1987;69(4):665-8.
  26. Zeilmaker GH, Alberda AT, van Gent I, Rijkmans CM, Drogendijk AC. Two pregnancies following transfer of intact frozen-thawed embryos. Fertil Steril. 1984;42(2):293-6.
  27. Temple-Smith PD, Southwick GJ, Yates CA, Trounson AO, de Kretser DM. Human pregnancy by in vitro fertilization (IVF) using sperm aspirated from the epididymis. J In Vitro Fert Embryo Transf. 1985;2(3):119-22.
  28. Cohen J, Simons RF, Fehilly CB, Fishel SB, Edwards RG, Hewitt J, et al. Birth after replacement of hatching blastocyst cryopreserved at expanded blastocyst stage. Lancet. 1985;1(8429):647.
  29. Wikland M, Enk L, Hamberger L. Transvesical and transvaginal approaches for the aspiration of follicles by use of ultrasound. Ann N Y Acad Sci. 1985;442:182-94.
  30. Strickler RC, Christianson C, Crane JP, Curato A, Knight AB, Yang V. Ultrasound guidance for hu man embryo transfer. Fertil Steril. 1985;43(1):54-61.
  31. Szöllösi D, Mandelbaum J, Plachot M, Salat-Baroux J, Cohen J. Ultrastructure of the human preovulatory oocyte. J In Vitro Fert Embryo Transf. 1986;3(4):232-42.
  32. Testart J, Lassalle B, Belaisch-Allart J, Hazout A, Forman R, Rainhorn JD, et al. High pregnancy rate after early human embryo freezing. Fertil Steril. 1986;46(2):268-72.
  33. Quinn P, Kerin JF, Warnes GM. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil Steril. 1985;44(4):493-8.
  34. Feichtinger W, Kemeter P. Transvaginal sector scan sonography for needle guided transvaginal follicle aspiration and other applications in gynecologic routine and research. Fertil Steril. 1986;45(5): 722-5.
  35. Rosenwaks Z, Veeck LL, Liu HC. Pregnancy following transfer of in vitro fertilized donated oocytes. Fertil Steril. 1986;45(3):417-20.
  36. Navot D, Laufer N, Kopolovic J, Rabinowitz R, Birkenfeld A, Lewin A, et al. Artificially induced endometrial cycles and establishment of pregnancies in the absence of ovaries. N Engl J Med. 1986;314(13):806-11.
  37. Devroey P, Braeckmans P, Smitz J, Van Waesberghe L, Wisanto A, Van Steirteghem A, et al. Pregnancy after translaparoscopic zygote intrafallopian transfer in a patient with sperm antibodies. Lancet. 1986;1(8493):1329.
  38. Chen C. Pregnancy after human oocyte cryopreservation. Lancet. 1986;1(8486):884-6.
  39. Laws-King A, Trounson A, Sathananthan H, Kola I. Fertilization of human oocytes by microinjection of a single spermatozoon under the zona pellucida. Fertil Steril. 1987;48(4):637-42.
  40. Trounson A, Peura A, Kirby C. Ultrarapid freezing: a new low-cost and effective method of embryo cryopreservation. Fertil Steril. 1987;48(5):843-50.
  41. Wilton LJ, Trounson AO. Biopsy of preimplantation mouse embryos: development of micromanipulated embryos and proliferation of single blastomeres in vitro. Biol Reprod. 1989;40(1):145-52.
  42. Patrizio P, Silber S, Ord T, Balmaceda JP, Asch RH. Two births after microsurgical sperm aspiration in congenital absence of vas deferens. Lancet. 1988;2(8624):1364.
  43. Ng SC, Bongso A, Ratnam SS, Sathananthan H, Chan CL, Wong PC, et al. Pregnancy after transfer of sperm under zona. Lancet. 1988;2(8614):790.
  44. Cohen J, Malter H, Fehilly C, Wright G, Elsner C, Kort H, et al. Implantation of embryos after partial opening of oocyte zona pellucida to facilitate sperm penetration. Lancet. 1988;2(8603):162.
  45. Handyside AH, Pattinson JK, Penketh RJ, Delhanty JD, Winston RM, Tuddenham EG. Biopsy of human preimplantation embryos and sexing by DNA amplification. Lancet. 1989;1(8634):347-9.
  46. Tadir Y, Wright WH, Vafa O, Ord T, Asch RH, Berns MW. Micromanipulation of sperm by a laser generated optical trap. Fertil Steril. 1989;52(5):870-3.
  47. Gonen Y, Casper RF, Jacobson W, Blankier J. Endometrial thickness and growth during ovarian stimulation: a possible predictor of implantation in in vitro fertilization. Fertil Steril. 1989;52(3):446-50.
  48. Gordts S, Roziers P, Campo R, Noto V. Survival and pregnancy outcome after ultrarapid freezing of human embryos. Fertil Steril. 1990;53(3):469-72.
  49. Handyside AH, Kontogianni EH, Hardy K, Winston RM. Pregnancies from biopsied human preim-plantation embryos sexed by Y-specific DNA amplification. Nature. 1990;344(6268):768-70.
  50. Cohen J, Elsner C, Kort H, Malter H, Massey J, Mayer MP, et al. Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum Reprod. 1990;5(1):7-13.
  51. Verlinsky Y, Ginsberg N, Lifchez A, Valle J, Moise J, Strom CM. Analysis of the first polar body: preconception genetic diagnosis. Hum Reprod. 1990;5(7):826-9.
  52. Gonen Y, Balakier H, Powell W, Casper RF. Use of gonadotropin-releasing hormone agonist to trigger follicular maturation for in vitro fertilization. J Clin Endocrinol Metab. 1990;71(4):918-22.
  53. Gonen Y, Jacobson W, Casper RF. Gonadotropin suppression with oral contraceptives before in vitro fertilization. Fertil Steril. 1990;53(2):282-7.
  54. Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril. 1991;55(1):109-13.
  55. Palanker D, Ohad S, Lewis A, Simon A, Shenkar J, Penchas S, et al. Technique for cellular microsurgery using the 193-nm excimer laser. Lasers Surg Med. 1991;11(6):580-6.
  56. Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet.
  57. Devroey P, van Steirteghem A, Mannaerts B, Bennink HC. Successful in-vitro fertilisation and embryo transfer after treatment with recombinant human FSH. Lancet. 1992;339(8802):1170.
  58. Patrizio P, Silber S, Ord T, Marello E, Balmaceda JP, Asch RH. Replacement of frozen embryos generated from epididymal spermatozoa: the first two pregnancies. Hum Reprod. 1992;7(5):652-3.
  59. Anguiano A, Oates RD, Amos JA, Dean M, Gerrard B, Stewart C, et al. Congenital bilateral absence of the vas deferens. A primarily genital form of cystic fibrosis. JAMA. 1992;267(13):1794-7.
  60. Emperaire JC, Ruffié A, Audebert AJ. [Ovulation induction by endogenous LH released by the administration of an LHRH agonist after follicular stimulation for in vitro fertilization]. J Gynecol Obstet Biol Reprod (Paris). 1992;21(5):489-94. French.
  61. Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, et al. Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia. Hum Reprod. 1995;10(6):1457-60.
  62. Patrizio P, Asch RH, Handelin B, Silber SJ. Aetiology of congenital absence of vas deferens: genetic study of three generations. Hum Reprod. 1993;8(2):215-20.
  63. Devroey P, Mannaerts B, Smitz J, Coelingh Bennink H, Van Steirteghem A. First established pregnancy and birth after ovarian stimulation with recombinant human follicle stimulating hormone (Org 32489). Hum Reprod. 1993;8(6):863-5.
  64. Trounson A, Wood C, Kausche A. In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril. 1994;62(2):353-62.
  65. Howles CM, Loumaye E, Giroud D, Luyet G. Multiple follicular development and ovarian steroid-genesis following subcutaneous administration of a highly purified urinary FSH preparation in pituitary desensitized women undergoing IVF: a multicentre European phase III study. Hum Reprod. 1994;9(3):424-30.
  66. Hull M, Corrigan E, Piazzi A, Loumaye E. Recombinant human luteinising hormone: an effective new gonadotropin preparation. Lancet. 1994;344(8918):334-5.
  67. Barnes FL, Crombie A, Gardner DK, Kausche A, Lacham-Kaplan O, Suikkari AM, et al. Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod. 1995;10(12):3243-7.
  68. Munné S, Sultan KM, Weier HU, Grifo JA, Cohen J, Rosenwaks Z. Assessment of numeric abnormalities of X, Y, 18, and 16 chromosomes in preim-plantation human embryos before transfer. Am J Obstet Gynecol. 1995;172(4 Pt 1):1191-9.
  69. Fishel S, Green S, Bishop M, Thornton S, Hunter A, Fleming S, et al. Pregnancy after intracytoplasmic injection of spermatid. Lancet. 1995;345(8965):1641-2.
  70. Dozortsev D, Rybouchkin A, De Sutter P, Qian C, Dhont M. Human oocyte activation following intracytoplasmic injection: the role of the sperm cell. Hum Reprod. 1995;10(2):403-7.
  71. Gil-Salom M, Romero J, Minguez Y, Rubio C, De los Santos MJ, Remohí J, et al. Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa. Hum Reprod. 1996;11(6):1309-13.
  72. Jurisicova A, Varmuza S, Casper RF. Programmed cell death and human embryo fragmentation. Mol Hum Reprod. 1996;2(2):93-8.
  73. Casper RF, Meriano JS, Jarvi KA, Cowan L, Lucato ML. The hypo-osmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril. 1996;65(5):972-6.
  74. Kahraman S, Polat G, Samli M, Sözen E, Ozgün OD, Dirican K, et al. Multiple pregnancies obtained by testicular spermatid injection in combination with intracytoplasmic sperm injection. Hum Reprod. 1998;13(1):104-10.
  75. Sun JG, Jurisicova A, Casper RF. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod. 1997;56(3):602-7.
  76. Cohen J, Scott R, Schimmel T, Levron J, Willadsen S. Birth of infant after transfer of anucleate donor oocyte cytoplasm into recipient eggs. Lancet. 1997;350(9072):186-7.
  77. Porcu E, Dal Prato L, Seracchioli R, Petracchi S, Fabbri R, Flamigni C. Births after transcervical gamete intrafallopian transfer with a falloposcopic delivery system. Fertil Steril. 1997;67(6):1175-7.
  78. Itskovitz-Eldor J, Kol S, Mannaerts B, Coelingh Bennink H. First established pregnancy after controlled ovarian hyperstimulation with recombinant follicle stimulating hormone and the gonado-trophin releasing hormone antagonist ganirelix (Org 37462). Hum Reprod. 1998;13(2):294-5.
  79. Gardner DK. Development of serum-free media for the culture and transfer of human blastocysts. Hum Reprod. 1998;13 Suppl 4:218-25.
  80. Palermo GD, Schlegel PN, Sills ES, Veeck LL, Zaninovic N, Menendez S, et al. Births after intracytoplasmic injection of sperm obtained by testicular extraction from men with nonmosaic Kline-felter's syndrome. N Engl J Med. 1998;338(9):588-90.
  81. Xu K, Shi ZM, Veeck LL, Hughes MR, Rosenwaks Z. First unaffected pregnancy using preimplantation genetic diagnosis for sickle cell anemia. JAMA. 1999;281(18):1701-6.
  82. Kuleshova L, Gianaroli L, Magli C, Ferraretti A, Trounson A. Birth following vitrification of a small number of human oocytes: case report. Hum Reprod. 1999;14(12):3077-9.
  83. Chian RC, Gülekli B, Buckett WM, Tan SL. Priming with human chorionic gonadotropin before retrieval of immature oocytes in women with infertility due to the polycystic ovary syndrome. N Engl J Med. 1999;341(21):1624, 1626.
  84. Buckett WM, Fisch P, Dean NL, Biljan MM, Tan SL. In vitro fertilization and intracytoplasmic sperm injection pregnancies after successful transport of oocytes by airplane. Fertil Steril. 1999;71(4):753-5.
  85. Nygren KG, Andersen AN. Assisted reproductive technology in Europe, 1999. Results generated from European registers by ESHRE. Hum Reprod. 2002;17(12):3260-74.
  86. Oktay K, Karlikaya G. Ovarian function after transplantation of frozen, banked autologous ovarian tissue. N Engl J Med. 2000;342(25):1919.
  87. Kojima K, Nomiyama M, Kumamoto T, Matsumoto Y, Iwasaka T. Transvaginal ultrasound-guided embryo transfer improves pregnancy and implantation rates after IVF. Hum Reprod. 2001;16(12):2578-82.
  88. Chian RC, Gülekli B, Buckett WM, Tan SL. Pregnancy and delivery after cryopreservation of zygotes produced by in-vitro matured oocytes retrieved from a woman with polycystic ovarian syndrome. Hum Reprod. 2001;16(8):1700-2.
  89. Abdul-Jalil AK, Child TJ, Phillips S, Dean N, Carrier S, Tan SL. Ongoing twin pregnancy after ICSI of PESA-retrieved spermatozoa into in-vitro matured oocytes: case report. Hum Reprod. 2001;16(7):1424-6.
  90. Belker AM, Swanson ML, Cook CL, Carrillo AJ, Yoffe SC. Live birth after sperm retrieval from a moribund man. Fertil Steril. 2001;76(4):841-3.
  91. De Boer K, McArthur S, Murray C, Jansen R. First live birth following blastocyst biopsy and PGD analysis. Reprod Biomed Online. 2002;4:35.
  92. Wells D, Escudero T, Levy B, Hirschhorn K, Delhanty JD, Munné S. First clinical application of comparative genomic hybridization and polar body testing for preimplantation genetic diagnosis of aneuploidy. Fertil Steril. 2002;78(3):543-9.
  93. Yoon TK, Kim TJ, Park SE, Hong SW, Ko JJ, Chung HM, et al. Live births after vitrification of oocytes in a stimulated in vitro fertilization-embryo transfer program. Fertil Steril. 2003;79(6):1323-6.
  94. Beckers NG, Macklon NS, Devroey P, Platteau P, Boerrigter PJ, Fauser BC. First live birth after ovarian stimulation using a chimeric long-acting human recombinant follicle-stimulating hormone (FSH) agonist (recFSH-CTP) for in vitro fertilization. Fertil Steril. 2003;79(3):621-3.
  95. Barash A, Dekel N, Fieldust S, Segal I, Schechtman E, Granot I. Local injury to the endometrium doubles the incidence of successful pregnancies in patients undergoing in vitro fertilization. Fertil Steril. 2003;79(6):1317-22.
  96. Donnez J, Dolmans MM, Demylle D, Jadoul P, Pirard C, Squifflet J, et al. Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet. 2004;364(9443):1405-10.
  97. Rao GD, Chian RC, Son WS, Gilbert L, Tan SL. Fertility preservation in women undergoing cancer treatment. Lancet. 2004;363(9423):1829-30.
  98. Chian RC, Buckett WM, Abdul Jalil AK, Son WY, Sylvestre C, Rao D, et al. Natural-cycle in vitro fertilization combined with in vitro maturation of immature oocytes is a potential approach in infertility treatment. Fertil Steril. 2004;82(6):1675-8.
  99. Gardner DK, Surrey E, Minjarez D, Leitz A, Stevens J, Schoolcraft WB. Single blastocyst transfer: a prospective randomized trial. Fertil Steril. 2004;81(3):551-5.
  100. Verlinsky Y, Rechitsky S, Sharapova T, Morris R, Taranissi M, Kuliev A. Preimplantation HLA testing. JAMA. 2004;291(17):2079-85.
  101. Xu K, Rosenwaks Z, Beaverson K, Cholst I, Veeck L, Abramson DH. Preimplantation genetic diagnosis for retinoblastoma: the first reported liveborn. Am J Ophthalmol. 2004;137(1):18-23.
  102. Meirow D, Levron J, Eldar-Geva T, Hardan I, Fridman E, Zalel Y, et al. Pregnancy after transplantation of cryopreserved ovarian tissue in a patient with ovarian failure after chemotherapy. N Engl J Med. 2005;353(3):318-21.
  103. Kokkali G, Vrettou C, Traeger-Synodinos J, Jones GM, Cram DS, Stavrou D, et al. Birth of a healthy infant following trophectoderm biopsy from blastocysts for PGD of beta-thalassaemia major. Hum Reprod. 2005;20(7):1855-9.
  104. Bedaiwy MA, Hussein MR, Biscotti C, Falcone T. Cryopreservation of intact human ovary with its vascular pedicle. Hum Reprod. 2006;21(12):3258-69.
  105. Ao A, Jin S, Rao D, Son WY, Chian RC, Tan SL. First successful pregnancy outcome after preimplantation genetic diagnosis for aneuploidy screening in embryos generated from naturalcycle in vitro fertilization combined with an in vitro maturation procedure. Fertil Steril. 2006; 85(5):1510.
  106. Son WY, Lee SY, Yoon SH, Lim JH. Pregnancies and deliveries after transfer of human blastocysts derived from in vitro matured oocytes in in vitro maturation cycles. Fertil Steril. 2007;87(6):1491-3.
  107. Heijnen EM, Eijkemans MJ, De Klerk C, Polinder S, Beckers NG, Klinkert ER, et al. A mild treatment strategy for in-vitro fertilisation: a randomised non-inferiority trial. Lancet. 2007;369(9563):743-9.
  108. Patrizio P, Gavish Z, Martel M, Azodi M, Silber S, Arav A. Whole human ovaries cryopreservation using a novel multi-gradient freezing device. Fertil Steril. 2007;88(1):S355.
  109. Son WY, Chung JT, Chian RC, Herrero B, Demirtas E, Elizur S, et al. A 38 h interval between hCG priming and oocyte retrieval increases in vivo and in vitro oocyte maturation rate in programmed IVM cycles. Hum Reprod. 2008;23(9):2010-6.
  110. Jones GM, Cram DS, Song B, Kokkali G, Pantos K, Trounson AO. Novel strategy with potential to identify developmentally competent IVF blastocysts. Hum Reprod. 2008;23(8):1748-59.
  111. Porcu E, Venturoli S, Damiano G, Ciotti PM, Notarangelo L, Paradisi R, et al. Healthy twins delivered after oocyte cryopreservation and bilateral ovariectomy for ovarian cancer. Reprod Biomed Online. 2008;17(2):265-7.
  112. Fishel S, Gordon A, Lynch C, Dowell K, Ndukwe G, Kelada E, et al. Live birth after polar body array comparative genomic hybridization prediction of embryo ploidy-the future of IVF? Fertil Steril. 2010;93(3):1006.
  113. Campbell A, Fishel S, Bowman N, Duffy S, Sedler M, Thornton S. Retrospective analysis of outcomes after IVF using an aneuploidy risk model derived from time-lapse imaging without PGS. Reprod Biomed Online. [Epub ahead of print].