Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Female Reproductive Hormones and Biomarkers of Oxidative Stress in Genital Chlamydia Infection in Tubal Factor Infertility

Authors
Augusta Nsonwu-Anyanwu 1
Mabel Charles-Davies 2
Victor Taiwo 3
Bin Li 4
Anthony Oni 5
Folashade Bello 6
1 Medical Lab., Science Department, College of Medical Sciences, University of Calabar, Calabar, Nigeria
2 Department of Chemical Pathology, University of Ibadan, Ibadan, Nigeria
3 Department of Veterinary Pathology, University of Ibadan, Ibadan, Nigeria
4 Unit of Molecular Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
5 Department of Medical Microbiology & Parasitology, University of Ibadan, Ibadan, Nigeria
6 Department of Obstetrics and Gynaecology, University of Ibadan, Ibadan, Nigeria
Abstract
Background: Genital Chlamydia infection (GCI) and the associated pathologies have been implicated in tubal infertility. Though the actual pathologic mechanisms are still uncertain, oxidative stress and other factors have been implicated. The purpose of the study was to determine the possible contribution of female reproductive hormones and biomarkers of oxidative stress in genital Chlamydial infection to tubal occlusion. Methods: This prospective case control study was carried out by recruiting 150 age matched women grouped into infertile Chlamydia positive women (n=50), fertile Chlamydia positive women (n=50) and fertile Chlamydia negative women as controls (n=50). High vaginal swabs and endocervical swabs were collected for screening Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Treponema pallidum, Staphylococcus aureus, and Candida albicans. Sera were collected for estimation of Chlamydia trachomatis antibody, female reproductive hormones [Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Oestradiol (E2), Progesterone (P4), Prolactin (PRL)] and biomarkers of oxidative stress [Total Antioxidant Capacity (TAC) and 8-hydroxyl-2-deoxyguanosine (8-OHdG)] by enzyme immunoassay (EIA). Data were analyzed using chi square, analysis of variance and LSD Post hoc to determine mean differences at p=0.05. Results: Among women with GCI, higher levels of LH and 8-OHdG were observed in infertile Chlamydia positive women compared to fertile Chlamydia positive women (p<0.05). Higher levels of LH and 8-OHdG and lower TAC levels were observed in infertile Chlamydia positive women compared to fertile Chlamydia negative controls (p<0.05). Conclusion: Mechanisms including oxidative DNA damage and reduced antioxidant capacity may be involved in the pathology of Chlamydia induced tubal damage.
Keywords
Chlamydia trachomatis
Female infertility
Hormone
oxidative stress
Tubal obstruction
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Journal of Reproduction & Infertility
Volume 16, Issue 2
Spring 2015
Pages 82-89