Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Effect of Acetylcholinesterase and Butyrylcholinesterase on Intrauterine Insemination, Contribution to Inflammations, Oxidative Stress and Antioxidant Status; A Preliminary Report

Authors
Lida Haghnazari 1
Asad Vaisi-Raygani 2
Farahnaz Keshvarzi 3
Farivar Ferdowsi 3
Massoud Goodarzi 4
Zohreh Rahimi 5
Hossin Baniamerian 6
Haidar Tavilani 7
Hadis Vaisi-Raygani 4
Hessam Vaisi-Raygani 4
Tayehbeh Pourmotabbed 8
1 Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran; Molecular Diagnostic Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran; Department of Clinical Bioc
3 Department of Obstetrics and Gynecology, Kermanshah University of Medical Sciences, Kermanshah, Iran
4 Molecular Diagnostic Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
5 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
6 Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
7 Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran
8 Department of Microbiology, Immunology and Biochemistry, University of Tennessee, Health Science Center, Tennessee, USA
Abstract
Background: Oxidative stress affects women fertility and influences on the sperm quality by alterating activities of cholinesterases, a molecular marker of stress-related infertility. The aim of the present study was to investigate the role of acetylcholinesterase (AChE), butyrylcholinesterase (BuChE) activities and phenotypes in patients with unexplained infertility (idiopathic). It’s possible association with inflammation marker C-reactive protein (CRP) and other oxidative stress markers, i.e. before and after intra uterine insemination (IUI). Methods: In this study, blood samples of 60 patients with unexplained infertility were collected the day before and 24 hr after IUI (between 8 AM and 9 AM after the overnight fasting) and activities of BuChE, AChE, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GpX) and serum levels of thiol proteins (TP), C-reactive protein (CRP), total antioxidant capacity (TAC) were measured. Statistical significance was assumed at p<0.05. Results: Before IUI, there was a significant (p=0.048) positive correlation between BuChE activity and plasma TAC and a significant difference in the CAT activity between various BuChE (UU and non-UU) phenotypes. However, after IUI, a significant negative correlation between the AChE activity and BuChE activity was found (p=0.045) and the level of RBC AChE activity was significantly reduced (382.4±163.19 vs. 586.7±384 IU/grHb, p=0.025). Meanwhile, after IUI, the activities of SOD (1568±847.5 IU/grHb vs. 1126±229.3, p=0.031) and CAT (310±53.4 IU/grHb vs. 338±73, p=0.025) were increased. Conclusion: This study suggests that decline in cholinesterases activities may be responsible for stimulation of oxidative stress and inflammation and reduction in fertility rates by IUI.
Keywords
Acetylcholinesterase (AChE)
Butyrylcholinesterase (BuChE)
Enzymatic and non
enzymatic antioxidant
Intrauterine insemination (IUI)
oxidative stress
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Journal of Reproduction & Infertility
Volume 17, Issue 3
Summer 2016
Pages 157-162