Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

The Study of Association Between Polymorphism of TNF-α Gene’s Promoter Region and Recurrent Pregnancy Loss

Authors
Roshanak Aboutorabi 1
Ehsan Behzadi 2
Mohammad Javad Sadegh 2
Seyed Pooriya Fatehi 2
Soroosh Semsarzadeh 2
Yasaman Zarrin 2
Mohammad Kazemi 3
Laleh Rafiee 4
Fatemeh Sadat Mostafavi 1
1 Infertility Laboratory, Beheshti Hospital, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Scien
2 School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: According to the literature review, polymorphisms of tumor necrosis factor alpha’s (TNF-α) promoter region are probably the genetic risk factors of recurrent pregnancy loss. This study has investigated five single nucleotide polymorphisms in the TNF-α gene’s promoter region to evaluate their relationship with recurrent pregnancy loss disorder. Methods: Blood samples were taken from 65 women with recurrent pregnancy loss (Case group) and 65 healthy women with a history of successful pregnancy (Control group). Polymerase chain reaction with high resolution melting (PCR-HRM) analysis was done to determine the promoter region of -308G/A, -850T/C, -238G/A, -1031T/C and -863A/C TNF-α polymorphisms. The data were assessed using logistic regression models. P values less than 0.05 were considered statistically significant. Results: Significant associations were found between recurrent pregnancy loss and -863C/A (p=0.000), -308G/A (p=0.045), and -238G/A (p=0.034) polymorphisms. TNF-α polymorphisms of -863C and -238G may be susceptible factors of recurrent pregnancy loss cases. The -308G polymorphism has an important role in maintaining pregnancy. Conclusion: The -863C/A and -238G/A TNF-α polymorphisms are possible genetic risk factors of recurrent pregnancy loss and might be its predictive markers.
Keywords
Polymorphism
Recurrent Pregnancy Loss
Tumor Necrosis Factor alpha
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Journal of Reproduction & Infertility
Volume 19, Issue 4
Autumn 2018
Pages 211-218