The Study of Association Between Polymorphism of TNF-α Gene’s Promoter Region and Recurrent Pregnancy Loss

Aboutorabi, Roshanak; Behzadi, Ehsan; Sadegh, Mohammad Javad; Fatehi, Seyed Pooriya; Semsarzadeh, Soroosh; Zarrin, Yasaman; Kazemi, Mohammad; Rafiee, Laleh; Mostafavi, Fatemeh Sadat

The Study of Association Between Polymorphism of TNF-α Gene’s Promoter Region and Recurrent Pregnancy Loss

Authors

Roshanak Aboutorabi ¹

Ehsan Behzadi ²

Mohammad Javad Sadegh ²

Seyed Pooriya Fatehi ²

Soroosh Semsarzadeh ²

Yasaman Zarrin ²

Mohammad Kazemi ³

Laleh Rafiee ⁴

Fatemeh Sadat Mostafavi ¹

¹ Infertility Laboratory, Beheshti Hospital, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Scien

² School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

³ Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

⁴ Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: According to the literature review, polymorphisms of tumor necrosis factor alpha’s (TNF-α) promoter region are probably the genetic risk factors of recurrent pregnancy loss. This study has investigated five single nucleotide polymorphisms in the TNF-α gene’s promoter region to evaluate their relationship with recurrent pregnancy loss disorder. Methods: Blood samples were taken from 65 women with recurrent pregnancy loss (Case group) and 65 healthy women with a history of successful pregnancy (Control group). Polymerase chain reaction with high resolution melting (PCR-HRM) analysis was done to determine the promoter region of -308G/A, -850T/C, -238G/A, -1031T/C and -863A/C TNF-α polymorphisms. The data were assessed using logistic regression models. P values less than 0.05 were considered statistically significant. Results: Significant associations were found between recurrent pregnancy loss and -863C/A (p=0.000), -308G/A (p=0.045), and -238G/A (p=0.034) polymorphisms. TNF-α polymorphisms of -863C and -238G may be susceptible factors of recurrent pregnancy loss cases. The -308G polymorphism has an important role in maintaining pregnancy. Conclusion: The -863C/A and -238G/A TNF-α polymorphisms are possible genetic risk factors of recurrent pregnancy loss and might be its predictive markers.

Keywords

Polymorphism

Recurrent Pregnancy Loss

Tumor Necrosis Factor alpha

Volume 19, Issue 4
Autumn 2018
Pages 211-218

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Journal of Reproduction & Infertility

The Study of Association Between Polymorphism of TNF-α Gene’s Promoter Region and Recurrent Pregnancy Loss

Volume 19, Issue 4Autumn 2018Pages 211-218

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Volume 19, Issue 4
Autumn 2018
Pages 211-218