Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

The First ongoing Pregnancy Following Comprehensive Aneuploidy Assessment Using a Combined Blastocenetesis, Cell Free DNA and Trophectoderm Biopsy Strategy

Authors
Jara Ben-Nagi 1
Rabi Odia 2
Xavier Gonzalez 2
Carleen Heath 2
Dhruti Babariya 3
Sioban SenGupta 4
Paul Serhal 1
Dagan Wells 5
1 Clinical Department, The Centre for Reproductive and Genetic Health, London, UK
2 Embryology Department, The Centre for Reproductive and Genetic Health, London, UK
3 Cooper Genomics, London, UK
4 Institute of Women’s Health, University College London, London, UK
5 Department of Women’s and Reproductive Health, University of Oxford, London, UK
Abstract
Background: The exact origin of cell-free DNA found in spent culture media or blastocoel fluid is currently unknown but with the potential to become an improved source of DNA for chromosomal analysis than trophectoderm biopsy samples, it provides a superior representation of the fetal genetic status. However, the genetic material contained within the blastocoel cavity may be more reliable to assessment of embryo euploidy in a clinical context than trophectoderm of cell-free DNA. Case Presentation: This is the first UK case report where all three sources of DNA were analyzed in a clinical setting on 29 th January 2018 at the Centre for Reproductive and Genetic Health, London, leading to an ongoing clinical pregnancy. Conclusion: The experience from this case report suggests that removal of blasto-coel fluid, sampling of spent culture media and trophectoderm biopsy can be carried out in parallel. Gathering genetic information from two to three independent samples of embryo DNA may provide enhanced diagnostic accuracy and may clarify cytogenetic status of mosaic embryos.
Keywords
biopsy
Blastocentesis
Cell free DNA
Journal of Reproduction & Infertility
Volume 20, Issue 1
Winter 2019
Pages 57-62