Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

The Effect of Preincubation Time and Myo-inositol Supplementation on the Quality of Mouse MII Oocytes

Authors
Fatemeh Mohammadi 1
Mahnaz Ashrafi 2
Zahra Zandieh 2
Mohammad Najafi 3
Behrooz Niknafs 4
Fatemeh Sadat Amjadi 5
Maryam Haghighi 1
1 Student Research Committee, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran; Anatomy Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran
2 Shahid Akbar Abadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences (IUMS), Tehran, Iran; Cellular and Molecular Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran
3 Biochemistry Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran
4 Anatomy Department, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5 Anatomy Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran; Shahid Akbar Abadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences (IUMS), Tehran, Iran
10.18502/jri.v21i4.4330
Abstract
Background: It is demonstrated that optimal preincubation time improves oocyte quality, fertilization potential and developmental rate. This study aimed to evaluate the effect of preincubation time in the simple and myo-inositol supplemented medium on the oocyte quality regarding oxidative stress and mitochondrial alteration. Methods: Cumulus oocyte complexes (COCs) retrieved from superovulated NMRI mice were divided in groups of 0, 4 and 8 hr preincubation time in the simple and 20 mmol/L myo-inositol supplemented media. Intracellular reactive oxygen species (H2O2), glutathione (GSH), mitochondrial membrane potential (MMP), ATP content, and mitochondrial amount were measured and analyzed in experimental groups. One-way ANOVA and Kruskal-Wallis were respectively used for parametric and nonparametric variables. Statistical significance was defined as p<0.05. Results: In comparison to control group, variables including ROS, GSH, mitochondrial amount, fertilization and developmental rates were significantly changed after 4 hr of preincubation in the simple medium, while MMP decreased following 8 hr of preincubation in the simple medium (p˂0.001). Preincubation of oocytes up to 8 hr in the simple medium could not decrease ATP content. For both 4 and 8 hr preincubation times, myo-inositole could decrease H2O2 and increase GSH and MMP levels and consequently could improve fertilization rate compared to oocytes preincubated in the simple culture. Conclusion: It seems that 4 hr or more preincubation time can decrease the oocyte quality and lead to reduced oocyte fertilization and developmental potential. Howevere, myo-inositol may prevent oocyte quality reduction and improve fertilization potential in comparision to the equivalent simple groups.
Keywords
Developmental Rate
Fertilization potential
Mitochondrial alteration
Myo
inositol supplement
Oocyte preincubation time
Oocyte quality
oxidative stress
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Journal of Reproduction & Infertility
Volume 21, Issue 4
Autumn 2020
Pages 259-268