Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Chromosomal Abnormalities in Couples with Primary and Secondary Infertility: Genetic Counseling for Assisted Reproductive Techniques (ART)

Authors
Subhadra Poornima 1
Swarnalatha Daram 1
Rama Devaki 2
Hasan Qurratulain 3
1 Department of Genetics and Molecular Medicine, Kamineni Life Sciences, Moulali, Hyderabad, India; Department of Genetics and Molecular Medicine, Kamineni Hospitals, Hyderabad, India
2 Department of Biochemistry, Kamineni Academy of Medical Sciences and Research Centre, Hyderabad, India
3 Department of Genetics and Molecular Medicine, Kamineni Hospitals, Hyderabad, India
10.18502/jri.v21i4.4331
Abstract
Background: World Health Organization estimates that 60-80 million couple worldwide currently suffer from infertility. Recurrent pregnancy loss (RPL) is also another major concern. Chromosomal rearrangements play a crucial role in primary and secondary infertility and RPL. Underlying genetic abnormalities like chromosomal abnormalities contribute to 5-10% of the reproductive failures. The aim of the study was to evaluate the chromosomal abnormalities in infertility and RPL cases to help obstetrician/fertility experts carry out risk assessment and provide appropriate assisted reproductive techniques for better management of the problem. Methods: Karyotyping was performed for 414 cases with the history of infertility and RPL over a period of one year. Samples were processed according to procedures of AGT cytogenetic laboratory manual. Results: Chromosomal abnormalities were observed in 15% of cases. Robertsonian translocation, reciprocal translocation, inversion, derivatives, marker chromosomes, mosaics, aneuploidy and polymorphic variants each contributed 2%, 3%, 3%, 13%, 2%, 10%, 6% and 61%, respectively. Conclusion: Evaluation of chromosomal abnormalities in couple is warranted prior to planning pregnancy especially for assisted reproductive management cases. Chromosomal analysis can be used as one of the diagnostic tools by OBG/IVF specialists in association with geneticist/genetic counselor for proper reproductive counseling and management.
Keywords
Banding
Culturing
Heterochromatin
Infertility
Inversion
Polymorphism
Translocation
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Journal of Reproduction & Infertility
Volume 21, Issue 4
Autumn 2020
Pages 269-274