Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

The First Successful Capacitation In Vitro Maturation of Oocyte in Indonesia: A Promising Strategy to Enhance IVF Accessibility for Infertile Couples

Authors
Andrew Kan 1
Nining Handayan 2
Arif Sofyan Aziz 1
Pitra Rahmawati 1
Arief Boediono 3
Ivan Sini 4
1 Morula IVF Jakarta Clinic, Jakarta, Indonesia
2 IRSI Research and Training Centre, Jakarta, Indonesia
3 Morula IVF Jakarta Clinic, Jakarta, Indonesia; IRSI Research and Training Centre, Jakarta, Indonesia; Faculty of Medicine, IPB University, Bogor, West Bengal, Indonesia
4 Morula IVF Jakarta Clinic, Jakarta, Indonesia; IRSI Research and Training Centre, Jakarta, Indonesia; Division of Biomedical Sciences, Faculty of Medicine and Nutrition, IPB University, West Java, Indonesia; Faculty of Medicine, I
Abstract
Background: The identification of c-type natriuretic peptide (CNP) as a key upstream regulator of cyclic GMP (cGMP) levels has paved the way for innova-tive in vitro maturation (IVM) techniques, the so-called biphasic CAPA-IVM (capacitation IVM) approach. This method uses CNP to prevent early meiosis progression and has demonstrated enhanced oocyte maturation rates. This report highlights the successful use of the CAPA-IVM method in Indonesia. Case Presentation: Immature cumulus-oocyte complexes (COCs) were successfully retrieved from an infertile woman aged 37 years without prior ovarian stimulation. The obtained COCs were incubated in CAPA medium for 24 hr and then transferred to a maturation medium containing amphiregulin, insulin, human serum albumin, and recombinant FSH. After 30 hr, all immature oocytes reached the metaphase II (MII) stage, as evidenced by the first polar body extrusion and confirmed through spindle formation. Conclusion: CAPA-IVM system enabled all retrieved immature oocytes to reach the MII stage without prior ovarian stimulation. This finding supports its feasibility as a simplified IVF approach with the potential to reduce treatment costs, particularly in resource-limited settings. However, further clinical evalua-tion is required to confirm its effectiveness.
Keywords
C
type oocytes
In vitro fertilization
In vitro oocyte maturation
Natriuretic peptide
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Journal of Reproduction & Infertility
Volume 27, Issue 1
Winter 2026
Pages 70-74