Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Sperm DNA oxidative damages in infertile men

Authors
Mohammad Rahbani Nobar 1
Nosratollah Zarghami 1
Jafar Nouroozzadeh 2
Yaghoub Deldar 3
Mohammad Nouri 1
Maerefat Ghaffari 4
1 Biochemistry Department, Tabriz Univarsity of Medical Sciences, Tabriz, Iran
2 Clinical Biochemistry Department, Faculty of Medicine, Urmia Medical Sciences University, Urmia, Iran
3 IVF Center of Kosar Hospital, Faculty of Medicine, Urmia Medical Sciences University, Urmia, Iran
4 Biochemistry Department, Tabriz Univarsity of Medical Sciences, Tehran, Iran; Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Abstract
Sperm DNA oxidative damage due to free radicals is a major contributing factor in a variety of human diseases, including male infertility. The aim of the present study was to compare the levels of sperm DNA oxidative damage in infertile (according to WHO criteria) and fertile men. Semen samples were obtained after 3 to 7 days of abstinence from 25 infertile and 23 fertile men who referred to infertility Center of Tabriz University of Medical Sciences. After semen analysis, sperm cells were separated from seminal fluid by percoll gradient centrifugation and kept at –20°C for next further analysis. Sperm DNA was extracted and its concentration and purity were determined by UV-spectrophotometer. DNA damage was studied by DNA Chromatography-mass spectrometry (GC/MS). Analysis of 8 hydroxy Guanine (8-OHG) as a marker of DNA oxidative damage by GC/MS was shown that rate of basal DNA oxidative damage in infertile group was 100-fold greater than fertile group. There was also correlation between semen parameter (morphology, motility and sperm count) and DNA oxidative damage. Result of this study showed that elevation of sperm DNA oxidative damage could be one of possible reasons for male infertility.
Keywords
8
hydroxy guanine
Antioxidant
Free radical
Infertility
Oxidative DNA damage
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Journal of Reproduction & Infertility
Volume 3, Issue 3
Summer 2002
Pages 65-74