Introduction: Assisted reproductive technologies have been used for the treatment of a consi-derable number of infertile couples. Conduction of several cycles of treatment, spending a lot of time, money and energy and the probable complications accompanying repeated anesthesia have made researchers find ways to predict the outcome of different methods used for the treatment of infertility. Male factor infertility is accountable for fifty percent of infertilities. Although semen analysis is an initial test to evaluate male fertility potentials but the results do not always predict fertilization outcomes. Sperm function tests have been suggested to predict the fertilization rate in ART treatment cycles. The objective of this study was to evaluate the diagnostic capabilities of double-stranded DNA in fertilization rate predictions. Materials & Methods: 100 infertile men were randomly selected. Based on WHO's 1999 cri-teria, semen analysis for each case was performed. DNA evaluation was performed by using Acridine orange. According to the fertilization rates (FR), the cases were divided into 3 groups: group I with FR>50%, group II with FR50%, FR