Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

The correlation between sperm DNA integrity and IVF success rate in infertile couples

Authors
Mahmoud Dehghani Ashkezari 1
Seyyed Mehdi Kalantar 2
Kazem Parivar 3
Abbas Aflatoonian 4
1 Department of Cellular and Molecular Biology, Molecular Research Center, Azad University, Yazd, Iran
2 Department of Reproduction & Genetics, Research & Clinical Center For Infertility, Yazd University of Medical Sciences & Health Services, Yazd, Iran
3 Department of Biology, Faculty of Sciences, Islamic Azad University, Science & Research Campus, Tehran, Iran
4 Department of Obstet . and Gynecol Research and Clinical Center for Infertility, Faculty of Medicine, Yazd Shahid Sadooghi University of Medical Sciences, Yazd, Iran
Abstract
Introduction: Assisted reproductive technologies have been used for the treatment of a consi-derable number of infertile couples. Conduction of several cycles of treatment, spending a lot of time, money and energy and the probable complications accompanying repeated anesthesia have made researchers find ways to predict the outcome of different methods used for the treatment of infertility. Male factor infertility is accountable for fifty percent of infertilities. Although semen analysis is an initial test to evaluate male fertility potentials but the results do not always predict fertilization outcomes. Sperm function tests have been suggested to predict the fertilization rate in ART treatment cycles. The objective of this study was to evaluate the diagnostic capabilities of double-stranded DNA in fertilization rate predictions. Materials & Methods: 100 infertile men were randomly selected. Based on WHO's 1999 cri-teria, semen analysis for each case was performed. DNA evaluation was performed by using Acridine orange. According to the fertilization rates (FR), the cases were divided into 3 groups: group I with FR>50%, group II with FR50%, FR
Keywords
Acridine orange
DNA normality
Double
stranded DNA
IVF
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Journal of Reproduction & Infertility
Volume 6, Issue 5
Winter 2006
Pages 505-512