Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Testis Specific Gene 10 expression in the testes of patients with non-obstructive azoospermia

Authors
Mahmood Aarabi 1
Haleh Soltanghoraee 2
Naser Amirjannati 2
Maerefat Ghaffari 2
Mohammad Reza Sadeghi 3
Mohammad Mehdi Akhondi 2
Mohammad Hossein Modarresi 4
1 Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
2 Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran; Avicenna Infertility Clinic, Avicenna Research Institute (ACECR), Tehran, Iran
3 Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran; Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
4 Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran; Nanobiotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran; Department of Medical Genetics, Faculty of Medicin
Abstract
Introduction: Disorders in the expression of any gene effective in spermatogenic pathway is known as a probable cause of non-obstructive azoospermia and male infertility. The way respon-sible genes for sperm motility are expressed can considerably affect male fertility. Recent studies show that TSGA10 gene is effective in the natural process of spermatogenesis as protein produced by this gene in mouse results in the production of the main structure of sperm tail. Up to now, no comprehensive studies have been done on the way this gene is expressed in the infertile's testical tissue. Materials & Methods: In this study, TSGA10 mRNA expression in testicular samples of 84 patients with non-obstructive azoospermia was investigated by semi-quantitative nested RT-PCR in Avesina Infertility Clinic during 2005-6. Moreover, expression levels of TSGA10 during spermatogenesis were evaluated using Johnsen’s method for histopathological scoring of the samples. For statistical analysis, SPSS software (Version 11.2) was used. The difference between gene expressions was done based on quantitative variables by the use of t-test and covariance analysis and α
Keywords
Azoospermia
Male infertility
spermatogenesis
Testicular biopsy
Testis specific gene 10 (TSGA10)
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Journal of Reproduction & Infertility
Volume 7, Issue 3
Autumn 2006
Pages 179-186