Journal of Reproduction & Infertility

Journal of Reproduction & Infertility

Association of ACE, PAI-1 and coagulation factor XIII gene polymorphisms with recurrent spontaneous abortion in Iranian patients

Authors
Haleh Soltanghoraee 1
Taktom Memariani 2
Mahmood Aarabi 2
Sedigheh Hantoushzadeh 3
Soheila Arefi 1
Mohsen Aarabi 4
Mohammad Mehdi Akhondi 5
Mohammad Hossein Modarresi 6
1 Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran; Avicenna Infertility Clinic, Avicenna Research Institute (ACECR), Tehran, Iran
2 Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
3 Faculty of Medical Tehran University of Medical Sciences, Tehran, Iran
4 Academic Unit of clinical Pharmacology ,University of Sheffield, Sheffield, United Kingdom
5 Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
6 Faculty of Medical Tehran University of Medical Sciences, Tehran, Iran; Nanobiotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
Abstract
Introduction: Considering the 5% prevalence of abortion in women, its devastating psycholo-gical effects on affected people’s lives and as tendency for clotting is one of the causes of recur-rent spontaneous abortion (RSA), this study was designed to compare plasminogen activator inhibitor-1 (PAI-1), angiotensin converting enzyme (ACE) and blood coagulation factor XIII (FXIII) gene polymorphisms in Iranian patients with RSA and healthy women. Materials & Methods: 120 patients with recurrent abortions (at least two) as cases and 112 healthy female controls without a history of abortion were entered into the study. In order to characterize PAI-1 (4G/5G), ACE (D/I) and FXIII (Val 34 Leu) polymorphisms, a polymerase chain reaction followed by digestion with restriction enzymes (PCR-RFLP) was designed. For the statistical analysis, SPSS software version 11.2 was used and t-test, Chi-square and Fisher’s exact tests were calculated. P-values
Keywords
Angiotensin Converting Enzyme
Coagulation factor XIII
Plasminogen activator inhibitor
1
Recurrent spontaneous abortion
Thrombophilia
Journal of Reproduction & Infertility
Volume 7, Issue 4
Winter 2007
Pages 324-330