The relationship between chromosomal abnormalities in unfertilized oocytes and female factors in IVF cycles

Kalantar, Seyyed Mehdi; Pilevarian, Ali Asghar; Moghaddasi, Seyyed Peyman

The relationship between chromosomal abnormalities in unfertilized oocytes and female factors in IVF cycles

Authors

Seyyed Mehdi Kalantar ¹

Ali Asghar Pilevarian ²

Seyyed Peyman Moghaddasi ²

¹ Department of Reproduction & Genetics, Research & Clinical Center For Infertility, Yazd University of Medical Sciences & Health Services, Yazd, Iran

² Faculty of Sciences, Isfahan Payam Noor University, Isfahan, Iran

Abstract

Introduction: Using Assisted Reproductive Techniques (ART) may result in the birth of a neonate and help solve the infertility problem. However, 10-15% of oocytes fail to fertilize and this is called a Total Fertilization Failure (TFF). This failure may impose financial burden on the infertile couples and be time consuming to IVF team. The aim of this study was to determine the relationship between chromosomal abnormalities of unfertilized eggs and female hormones, FSH and LH. Materials & Methods: Unfertilized oocytes from IVF treatment cycles were prepared by Tar-kowski method for chromosomal studies and after staining by Giemsa method, they were chro-mosomally analyzed. For hormonal evaluations of FSH and LH, ELISA was used. The results were analyzed by ANOVA and correlation coefficient calculations through using SPSS software and p-values

Keywords

Chromosomal abnormalities

Female hormones

Infertility

IVF

Unfertilized oocytes

Edirisinghe WR, Murch AR, Yovich JL. Cytogenetic analysis of human oocytes and embryos in an In vitro fertilization programme. Hum Reprod. 1992;7:230-6.

Bedford JM, Kim HH. Sperm/egg binding patterns and oocytes cytology in retrospective analysis of fertili-zation failure In vitro. Hum Reprod. 1993;8:453-63.

Wall MB, Marks K, Smith TA. Cytogenetic and fluore-scent in-situ hybridization chromosomal studies on In-vitro fertilized and intracytoplasmic sperm injected failed-fertilized. University Press, Cambridge, UK. 1996;pp:235-242.

Abruzzo MA, Hassold TA. Etiology of nondisjunction in humans. Environ Mol. Mutagen 1995;25(Suppl. 26): 38-47.

Kalantar SM, Lenton EA, Barratt CLR. Sperm function tests and fertilization failure following IVF. In Human sperm acrosome reaction. Fenichel P, Parinaud J, (Editors). Colloque INSERM/John Libbey Eurotext Ltd. 1995;236:pp.439-440.

Kalantar SM, Lenton EA. Evaluating the ability of bio-logical substancesfor induction of acrosome reaction in normospermic samples. Midd East Ferti Soci J. 2000; 5:2;71-78.

Kalantar SM, Lenton EA, Brewis I, Barratt C, Moore H. Fertilization failure of IVF predictive power of SFT. Andrologia. 1998;30(4-5):180-181.

Plachot M. Oocyte- genetic aspects. In Grudzinskas J G, Yovich JL (Editors). Gametes- The Oocyte. Camb-ridge University Press, Cambridge. 1995;pp:95-107.

Nakaoka Y, Okamoto E, Miharu1 N, Ohama K. Chro-mosome analysis in human oocytes remaining unferti-lized after In-vitro insemination: effect of maternal age and fertilization rate. Hum Reprod. 1998;13:419-424.

Ma S, Robinson W, Ryan Lam, Basil Ho Yuen. Mater-nal origin of monosomy 21 derived from ICSI: Case report. Hum Reprod. 2001:16;1100-3.

Macas E, Floresheim Y, Hotz E, Imthurn B, Keller PJ, Walt H. Abnormal chromosomal arrangements in human oocytes. Hum Reprod. 1990;5:703-7.

Pellestor F, Anahory T, Hamamah S. The chromoso-mal analysis of human oocytes. An overview of estab-lished procedures. Hum Reprod Update. 2004;1-18.

Tarin JJ, Gomez E, Sampaio M. Cytogenetic analysis of human oocytes from fertile women. Hum Reprod. 1991;8:1100-3.

Kamiguchi Y, Rosenbusch B, Sterizik K, Mikamo K. Chromosome analysis of unfertilized human oocytes prepared by a gradual fixation-air human oocytes. Hum Reprod. 1993;11:2230-8.

Almeida PA, Bolton VN. The relationship between chromosomal abnormalities in the human oocytes and fertilization in vivo. Hum Reprod. 1994;9:343-6.

Sengoku K, Tamate K, Takuma N, Yoshida T, Goishi K, Ishikawa M. The chromosomal normality of unferti-lizes oocytes from patients with polycystic ovarian syndrome Hum Reprod. 1997;12(3):474-7.

Agarwal A, Said TB, Bedaiw MAy, Banerjee J, Alva-rez JG. Oxidative stress in an assisted reproductive techniques setting. Fertil Steril. 2006;86: 503-14.

Kalantar SM, Ebrahimi A, Fazli H, Soleimani M, Aflatoonian A. The chromosomal abnormality of un-fertilized oocytes in In Vitro Fertilization program. I.J. R.M. 2003;1(1):32-36.

World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cer-vical Mucus Interaction. 4th Edition. Cambridge. 1999.

Aflatounian A, Karimzadeh MA, Dehghani R, Kalan-tar SM, Soliman M. The role of aspiration of half ovarian follicles prior to admistration of hCG or GnRH -a for prevention of severe OHSS in ART programs. Midd. East. Fertil Soci. 2000;5(1):73-5.

Tarkowski AK. An air-drying method for chromo-some preparation from mouse eggs. Cytogenetics. 1996;5:394-400.

Mitelman F. An International System for Human Cytogentic Nomenclature. Karger. Switzerland. 1995

Bedford JM, Kim HH. Sperm/egg binding patterns and oocytes cytology in retrospective analysis of fertiliza-tion failure in vitro. Hum Reprod. 1993;8:453-63.

Wall MB, Marks K, Smith TA, Gearou CM, Magg-leton-Harris AL. Cytogenetic and fluorescent in-situ hybridization chromosomal studies on in-vitro ferti-lized and intracytoplasmic sperm injected ‘failed-fertilized’ human oocyte. Hum Reprod. 1996;11(10): 2230-8.

Mozdarani H, Aghaei F. Cytogenetics analysis of failed-fertilized oocytes from Iranian infertile women after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) procedures. MEFSJ. 2001;6(3): 216-225.

Dyban A, De Sutter P, Verlinsky Y. Preimplantation cytogenetic analysis. In Verlinsky Y, Kuleiy AM (Edi-tors). Preimplantation Diagnosis of Genetic Disease. 1993; Wiley-Liss New York, pp.98.

Nakaoka Y, Okamoto E, Miharu N, Ohama K. Choro-mosome analysis in human oocytes remaining unferti-lizes after in-vitro insemination: effect of maternal age and fertilization rate. Hum Reprod. 1998; 13(2):419-24.

Lim AST, Ho ATN, Tsakok MFH. Chromosomes of oocytes failing in-vitro fertilization. Hum Reprod. 1995;10:2570-5.

Pellestor F, Andréo B, Arnal F, Humeau C, Demaille J. Mechanisms of non-disjunction in human female meiosis: the co-existence of two modes of malsegre-gation evidenced by the karyotyping of 1397 in-vitro unfertilized oocytes. Hum Reprod. 2002;17: 2134-45.

Smith R, Walker L, Cobo AC, Contribution of chro-mosomal abnormalities to in vitro fertilization failures. Rev Med Chil. 1998;126(5):511-9.

Zenzes MT, Casper RF. Cytogenetics of human oo-cytes, zygotes and embryos after in vitro fertilization. Hum Genet. 1992;88:367-37.

Angell R. First-meiotic-division nondisjunction in hu-man oocytes. Am J Hum Genet. 1997;61:23-32.

Pellestor F. Frequency and distribution of aneuploidy in human female gametes. Hum Genet. 1991;86:283-8.

Pellestor F, Andréo B, Arnal F, Humeau C, Demaille J. Maternal aging and chromosomal abnormalities: new data drawn from in vitro unfertilized human oocytes. Hum Genet. 2003;112:195-203.

Takehiro I, Masayuki K, Hiroyuki A, Yutaka S, Hiro-yoshi H. Growth, antrum formation and estradiol pro-duction of bovine preantral follicles cultured in a serum-free medium. Biol Reprod. 2002;67:1099-105.

Lin YH, Hwang JL, Huang LW, Mu SC, Seow KM, Chung J, et al. Combination of FSH priming and hCG priming for in-vitro maturation of human oocytes. Hum Reprod. 2003;18:1632-36.

Gras L, McBain J, Trounson A, Kola I. The incidence of chromosome aneuploidies in stimulated and unsti-mulated (natural) uninseminated human oocytes. Hum Reprod. 1992;7:1396-401.

Gomes CM, Raineki C, de Paula PR, Severino GS, Helena CVV, Anselmo-Franci JA, et al. Neonatal handling and reproductive function in female rats. J Endocrinol. 2005;184(2):435-45.